FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-ALK-1/ACVRL1 antibody (AAA19242).Overlay histogram showing MCF-7 cells stained with AAA19242 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALK-1/ACVRL1 Antibody (AAA19242, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit ALK-1/ACVRL1 Polyclonal Antibody | anti-ACVRL1 antibody
Anti-ALK-1/ACVRL1 Antibody
; activin A receptor like type 1; anti-ACVRL1 antibody
IHC-P: 2-5ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-ALK-1/ACVRL1 antibody (AAA19242).Overlay histogram showing MCF-7 cells stained with AAA19242 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALK-1/ACVRL1 Antibody (AAA19242, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-ALK-1/ACVRL1 antibody (AAA19242).Overlay histogram showing MCF-7 cells stained with AAA19242 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALK-1/ACVRL1 Antibody (AAA19242, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IHC (Immunohistochemistry)
(Figure 5. IHC analysis of ALK-1/ACVRL1 using anti-ALK-1/ACVRL1 antibody (AAA19242).ALK-1/ACVRL1 was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ALK-1/ACVRL1 Antibody (AAA19242) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
IHC (Immunohistochemistry)
(Figure 5. IHC analysis of ALK-1/ACVRL1 using anti-ALK-1/ACVRL1 antibody (AAA19242).ALK-1/ACVRL1 was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ALK-1/ACVRL1 Antibody (AAA19242) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of ALK-1/ACVRL1 using anti-ALK-1/ACVRL1 antibody (AAA19242).ALK-1/ACVRL1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ALK-1/ACVRL1 Antibody (AAA19242) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of ALK-1/ACVRL1 using anti-ALK-1/ACVRL1 antibody (AAA19242).ALK-1/ACVRL1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ALK-1/ACVRL1 Antibody (AAA19242) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
IHC (Immunohistochemistry)
(Figure 3. IHC analysis of ALK-1/ACVRL1 using anti-ALK-1/ACVRL1 antibody (AAA19242).ALK-1/ACVRL1 was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ALK-1/ACVRL1 Antibody (AAA19242) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
IHC (Immunohistochemistry)
(Figure 3. IHC analysis of ALK-1/ACVRL1 using anti-ALK-1/ACVRL1 antibody (AAA19242).ALK-1/ACVRL1 was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ALK-1/ACVRL1 Antibody (AAA19242) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
IHC (Immunohistochemistry)
(Figure 2. IHC analysis of ALK-1/ACVRL1 using anti-ALK-1/ACVRL1 antibody (AAA19242).ALK-1/ACVRL1 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ALK-1/ACVRL1 Antibody (AAA19242) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
IHC (Immunohistochemistry)
(Figure 2. IHC analysis of ALK-1/ACVRL1 using anti-ALK-1/ACVRL1 antibody (AAA19242).ALK-1/ACVRL1 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ALK-1/ACVRL1 Antibody (AAA19242) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
WB (Western Blot)
(Figure 1. Western blot analysis of ALK-1/ACVRL1 using anti-ALK-1/ACVRL1 antibody (AAA19242).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human HL-60 whole cell lysatesLane 2: human THP-1 whole cell lysatesLane 3: rat brain tissue lysatesLane 4: rat heart tissue lysatesLane 5: mouse brain tissue lysatesLane 6: mouse kidney tissue lysatesLane 7: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ALK-1/ACVRL1 antigen affinity purified polyclonal antibody (Catalog # AAA19242) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for ALK-1/ACVRL1 at approximately 55KD. The expected band size for ALK-1/ACVRL1 is at 55KD.)
WB (Western Blot)
(Figure 1. Western blot analysis of ALK-1/ACVRL1 using anti-ALK-1/ACVRL1 antibody (AAA19242).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human HL-60 whole cell lysatesLane 2: human THP-1 whole cell lysatesLane 3: rat brain tissue lysatesLane 4: rat heart tissue lysatesLane 5: mouse brain tissue lysatesLane 6: mouse kidney tissue lysatesLane 7: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ALK-1/ACVRL1 antigen affinity purified polyclonal antibody (Catalog # AAA19242) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for ALK-1/ACVRL1 at approximately 55KD. The expected band size for ALK-1/ACVRL1 is at 55KD.)
2. Abdalla, S. A., Letarte, M. Hereditary haemorrhagic telangiectasia: current views on genetics and mechanisms of disease. J. Med. Genet. 43: 97-110, 2006.
3. Abdalla, S. A., Pece-Barbara, N., Vera, S., Tapia, E., Paez, E., Bernabeu, C., Letarte, M. Analysis of ALK-1 and endoglin in newborns from families with hereditary hemorrhagic telangiectasia type 2. Hum. Molec. Genet. 9: 1227-1237, 2000.
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Product Notes
The ACVRL1 acvrl1 (Catalog #AAA19242) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ALK-1/ACVRL1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ALK-1/ACVRL1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA (Direct ELISA). WB: 0.25-0.5ug/ml|Human, Mouse, Rat| IHC-P: 2-5ug/ml|Human| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the ACVRL1 acvrl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALK-1/ACVRL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What applications is this ACVRL1 antibody validated for (WB, IHC, IF)?
This ACVRL1 polyclonal antibody is validated for multiple applications, including Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry, and ELISA, making it versatile for various research techniques.
Which species does this ACVRL1 polyclonal antibody detect?
The ACVRL1 polyclonal antibody detects the protein in human, mouse, and rat samples, with tested reactivity confirmed in human tissues and cited reactivity across multiple mammalian species.
What is the recommended dilution range for common assays?
Recommended dilutions vary by application: Western Blot 1:500–1:1000, IHC 1:50–1:500, and Flow Cytometry 0.25 µg per 10⁶ cells. Titration in your specific system is recommended for optimal results.
Is this antibody specific to ACVRL1 or does it show cross-reactivity?
The ACVRL1 antibody demonstrates high specificity to ACVRL1 with minimal cross-reactivity. Some formulations show approximately 10% cross-reactivity with recombinant mouse ALK-1 and less than 1% with other Activin receptors.
Is the antibody suitable for detecting endogenous ACVRL1 in tissue samples?
Yes, the antibody effectively detects endogenous ACVRL1 in various tissue samples, including human heart, placenta, and lymphoid tissues, as validated by immunohistochemistry and Western blot analysis.
What storage conditions are required to maintain antibody stability?
Store the ACVRL1 antibody at –20°C in PBS buffer containing glycerol and sodium azide. It remains stable for one year after shipment; avoid freeze-thaw cycles by aliquoting.
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