Rabbit anti-Human ALK Polyclonal Antibody | anti-ALK antibody

ALK (Phospho-Tyr1604) Antibody

Cat. Num. AAA304630

• Gene Names: ALK; CD246; NBLST3
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: ALK, Antibody; ALK (Phospho-Tyr1604) Antibody; ALK tyrosine kinase receptor precursor; Anaplastic lymphoma kinase; CD246; EC 2.7.10.1; kinase ALK; anti-ALK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of ALK only when phosphorylated at tyrosine 1604.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1620

• Applicable Applications for anti-ALK antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: peptide
• Immunogen Description: Peptide sequence around phosphorylation site of tyrosine 1604 (G-H-Y(p)-E-D) derived from Human ALK.
• Modification: Phospho-Tyr1604
• Target Name: ALK
• Preparation and Storage: Store at -20 degree C

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cells, using ALK (Phospho-Tyr1604) antibody. The picture on the right is treated with the synthesized peptide.)

IHC (Immunohistochemisry)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ALK (Phospho-Tyr1604) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot)

(Western blot analysis of extracts from cos-7 cells (Lane 2), using ALK (Phospho-Tyr1604) Antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot)

(Western blot analysis of extracts from HepG2 cells, using ALK (Phospho-Tyr1604) antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-ALK antibody

Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.

Product Categories/Family for anti-ALK antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 29029632
• NCBI GeneID: 238
• NCBI Accession #: NP_004295.2
• NCBI GenBank Nucleotide #: NM_004304.4
• UniProt Accession #: Q9UM73; Q4ZFX9; Q53QQ6; Q53RZ4; Q59FI3; Q9Y4K6
• Molecular Weight: 176,442 Da
• NCBI Official Full Name: ALK tyrosine kinase receptor
• NCBI Official Synonym Full Names: anaplastic lymphoma receptor tyrosine kinase
• NCBI Official Symbol: ALK
• NCBI Official Synonym Symbols: CD246; NBLST3
• NCBI Protein Information: ALK tyrosine kinase receptor
• UniProt Protein Name: ALK tyrosine kinase receptor
• UniProt Gene Name: ALK
• UniProt Entry Name: ALK_HUMAN