FCM/FACS (Flow Cytometry)
(ALOX12B Antibody (C-term)(AAA283777) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ALOX12B Polyclonal Antibody | anti-ALOX12B antibody
ALOX12B Antibody (C-term)
Gene Names
ALOX12B; ARCI2; 12R-LOX
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ALOX12B, Antibody; ALOX12B Antibody (C-term); Arachidonate 12-lipoxygenase; 12R-type; 12R-LOX; 12R-lipoxygenase; 11311-; Epidermis-type lipoxygenase 12; ALOX12B; anti-ALOX12B antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ALOX12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 622-652 amino acids from the C-terminal region of human ALOX12B.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
622-652
Sequence Length
701
Applicable Applications for anti-ALOX12B antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Catalyzes the regio and stereo-specific incorporation of a single molecule of dioxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed: , PubMed: , PubMed: ). In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins (PubMed: ). Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss (PubMed: ). May also play a role in the regulation of the expression of airway mucins (PubMed:22441738).
Cellular Location
Cytoplasm {ECO:0000255|PROSITE-ProRule:PRU00726 }. Cytoplasm, perinuclear region
Tissue Location
Expressed in B-cells, hair follicles, foreskin keratinocytes and adult skin. Also expressed in psoriatic tissue
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ALOX12B Antibody (C-term)(AAA283777) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ALOX12B Antibody (C-term)(AAA283777) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(ALOX12B antibody (C-term) (AAA283777) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ALOX12B antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(ALOX12B antibody (C-term) (AAA283777) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ALOX12B antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(ALOX12B Antibody (C-term) (AAA283777) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ALOX12B antibody detected the ALOX12B protein (arrow).)
WB (Western Blot)
(ALOX12B Antibody (C-term) (AAA283777) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ALOX12B antibody detected the ALOX12B protein (arrow).)
Related Product Information for anti-ALOX12B antibody
This gene encodes an enzyme involved in the converstion of
arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in
this gene are associated with nonbullous congenital ichthyosiform
erythroderma.
arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in
this gene are associated with nonbullous congenital ichthyosiform
erythroderma.
Product Categories/Family for anti-ALOX12B antibody
References
Han, S., et al. Hum. Immunol. 71(7):727-730(2010)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010)
Vahlquist, A., et al. J. Invest. Dermatol. 130(2):438-443(2010)
Agarwal, S., et al. Exp. Dermatol. 18(11):939-946(2009)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 18(5):1651-1658(2009)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010)
Vahlquist, A., et al. J. Invest. Dermatol. 130(2):438-443(2010)
Agarwal, S., et al. Exp. Dermatol. 18(11):939-946(2009)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 18(5):1651-1658(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
80356
NCBI Official Full Name
arachidonate 12-lipoxygenase, 12R-type
NCBI Official Synonym Full Names
arachidonate 12-lipoxygenase, 12R type
NCBI Official Symbol
ALOX12B
NCBI Official Synonym Symbols
ARCI2; 12R-LOX
NCBI Protein Information
arachidonate 12-lipoxygenase, 12R-type
UniProt Protein Name
Arachidonate 12-lipoxygenase, 12R-type
UniProt Gene Name
ALOX12B
UniProt Synonym Gene Names
12R-LOX; 12R-lipoxygenase
UniProt Entry Name
LX12B_HUMAN
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Product Notes
The ALOX12B alox12b (Catalog #AAA283777) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 622-652. The ALOX12B Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ALOX12B can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ALOX12B alox12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALOX12B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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