Rabbit anti-Human Alpha B Crystallin Polyclonal Antibody

Anti-Alpha B Crystallin Antibody, Rabbit Polyclonal

Cat. Num. AAA258802

• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Alpha B Crystallin, Antibody; Anti-Alpha B Crystallin Antibody, Rabbit Polyclonal; crystallin, alpha B; Anti-CMD1II Antibody; Anti-CRYA2 Antibody; Anti-CTPP2 Antibody; Anti-CTRCT16 Antibody; Anti-HEL-S-101 Antibody; Anti-HSPB5 Antibody; Anti-MFM2 Antibody; anti-Alpha B Crystallin antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Alpha B Crystallin
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-Alpha B Crystallin antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
• Immunogen: A synthetic peptide corresponding to the center region of the Human Alpha B Crystallin
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Alpha B Crystallin, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze !

IHC (Immunohistochemisry)

(Immunochemical staining of human CRYAB in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human CRYAB in human muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IF (Immunofluorescence)

(Immunofluorescence staining of Alpha B Crystallin in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human Alpha B Crystallin polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Related Product Information for anti-Alpha B Crystallin antibody

Alpha B-Crystallin (CRYAB) is purported to be a metastasis suppressor protein, and lack or lower CRYAB expression is a prognostic biomarker for several types of cancer, such as that of the prostate and head and neck. Mutations in alpha-B crystallin gene (CRYAB) have been described to cause congenital cataracts, dilated cardiomyopathy and myofibrillar myopathy. Mutations in the alphaB-crystallin (CRYAB) gene, encoding a small heat shock protein with chaperone function, are a rare cause of myofibrillar myopathy with autosomal-dominant inheritance, late-onset and moderate severity. Mutations in the CRYAB gene, encoding alpha-B crystallin, cause distinct clinical phenotypes including isolated posterior polar cataract, myofibrillar myopathy, cardiomyopathy, or a multisystemic disorder combining all these features. CRYAB may be identified as a novel prognostic marker and targeting CRYAB may provide a promising strategy for NSCLC treatment.