IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human kidney using AAA230308 at dilution 1:100)
Rabbit Alpha-crystallin B chain Polyclonal Antibody | anti-CRYAB antibody
Rabbit anti-human Alpha-crystallin B chain polyclonal Antibody
Gene Names
CRYAB; MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
Reactivity
Human, mouse, rat
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Alpha-crystallin B chain, Antibody; Rabbit anti-human Alpha-crystallin B chain polyclonal Antibody; Alpha(B)-crystallin; Heat shock protein beta-5; HspB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component; CRYAB; CRYA2; anti-CRYAB antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
175
Applicable Applications for anti-CRYAB antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human Alpha-crystallin B chain protein(1-175aa)
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-22391 / sc-22744
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human kidney using AAA230308 at dilution 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human kidney using AAA230308 at dilution 1:100)
WB (Western Blot)
(Western blotAll lanes: Alpha-crystallin B chain antibody at 2ug/mlLane 1:293T whole cell lysateLane 2:mouse eye tissueLane 3:rat lung tissueLane 4:rat heart tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 20kDaObserved band size: 20kDa)
WB (Western Blot)
(Western blotAll lanes: Alpha-crystallin B chain antibody at 2ug/mlLane 1:293T whole cell lysateLane 2:mouse eye tissueLane 3:rat lung tissueLane 4:rat heart tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 20kDaObserved band size: 20kDa)
Related Product Information for anti-CRYAB antibody
May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
Product Categories/Family for anti-CRYAB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,159 Da
NCBI Official Full Name
alpha-crystallin B chain
NCBI Official Synonym Full Names
crystallin alpha B
NCBI Official Symbol
CRYAB
NCBI Official Synonym Symbols
MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
NCBI Protein Information
alpha-crystallin B chain
UniProt Protein Name
Alpha-crystallin B chain
UniProt Gene Name
CRYAB
UniProt Synonym Gene Names
CRYA2; HSPB5; HspB5
UniProt Entry Name
CRYAB_HUMAN
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Product Notes
The CRYAB cryab (Catalog #AAA230308) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Alpha-crystallin B chain polyclonal Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha-crystallin B chain can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CRYAB cryab for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha-crystallin B chain, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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