WB (Western Blot)
(Anti-INA rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse brain tissue lysateLane B: rat brain tissue lysateLane C: SHSY5Y Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human alpha Internexin/INA Polyclonal Antibody | anti-INA antibody
Anti-alpha Internexin/INA Antibody, Rabbit Polyclonal
Gene Names
INA; NEF5; NF-66; TXBP-1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
alpha Internexin/INA, Antibody; Anti-alpha Internexin/INA Antibody, Rabbit Polyclonal; INA Antibody, Rabbit PAb, Antigen Affinity Purified; internexin neuronal intermediate filament protein, alpha; Anti-NEF5 Antibody; Anti-NF-66 Antibody; Anti-TXBP-1 Antibody; anti-INA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human alpha Internexin/INA
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-INA antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human INA
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human INA, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-INA rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse brain tissue lysateLane B: rat brain tissue lysateLane C: SHSY5Y Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-INA rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse brain tissue lysateLane B: rat brain tissue lysateLane C: SHSY5Y Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(INA was immunoprecipitated using:Lane A:0.5 mg SH-SY5Y Whole Cell Lysate2 uL anti-INA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-INA rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size: 55 kDa)
IP (Immunoprecipitation)
(INA was immunoprecipitated using:Lane A:0.5 mg SH-SY5Y Whole Cell Lysate2 uL anti-INA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-INA rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size: 55 kDa)
Related Product Information for anti-INA antibody
INA (Internexin Neuronal Intermediate Filament Protein Alpha) is a Protein Coding gene. INA belongs to the intermediate filament family. Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. INA is a class-IV neuronal intermediate filament that can self-assemble. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges. Diseases associated with INA include Gastroenteropancreatic Neuroendocrine Neoplasm and Medulloepithelioma.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,391 Da
NCBI Official Full Name
alpha-internexin
NCBI Official Synonym Full Names
internexin neuronal intermediate filament protein, alpha
NCBI Official Symbol
INA
NCBI Official Synonym Symbols
NEF5; NF-66; TXBP-1
NCBI Protein Information
alpha-internexin; alpha-Inx; neurofilament 5 (66kD); 66 kDa neurofilament protein; neurofilament-66, tax-binding protein
UniProt Protein Name
Alpha-internexin
UniProt Gene Name
INA
UniProt Synonym Gene Names
NEF5; Alpha-Inx; NF-66
UniProt Entry Name
AINX_HUMAN
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Product Notes
The INA ina (Catalog #AAA254277) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-alpha Internexin/INA Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's alpha Internexin/INA can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the INA ina for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha Internexin/INA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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