FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-Alpha SNAP/NAPA antibody (AAA127279).Overlay histogram showing 293T cells stained with AAA127279 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha SNAP/NAPA Antibody (AAA127279, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit anti-Human Alpha SNAP/NAPA Polyclonal Antibody | anti-AlphaSNAP/NAPA antibody
Anti-Alpha SNAP/NAPA Antibody Picoband
Gene Names
NAPA; SNAPA
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Alpha SNAP/NAPA, Antibody; Anti-Alpha SNAP/NAPA Antibody Picoband; Long-chain-fatty-acid--CoA ligase 5; Sodium- and chloride-dependent GABA transporter 1; GAT-1; Solute carrier family 6 member 1; SLC6A1; GABATR, GABT1, GAT1; anti-AlphaSNAP/NAPA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Expressed in many tissues, highest levels in skeletal muscle.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-AlphaSNAP/NAPA antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Alpha SNAP/NAPA, which shares 95.7% amino acid (aa) sequence identity with mouse and rat NAPA.
Subcellular Localization
Cell membrane, Multi-pass membrane protein. Membrane, Multi-pass membrane protein. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons (By similarity).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-Alpha SNAP/NAPA antibody (AAA127279).Overlay histogram showing 293T cells stained with AAA127279 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha SNAP/NAPA Antibody (AAA127279, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-Alpha SNAP/NAPA antibody (AAA127279).Overlay histogram showing 293T cells stained with AAA127279 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha SNAP/NAPA Antibody (AAA127279, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of Alpha SNAP/NAPA using anti-Alpha SNAP/NAPA antibody (AAA127279).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human 293T whole cell lysates,Lane 2: human MCF-7 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human K562 whole cell lysates,Lane 5: human THP-1 whole cell lysates,Lane 6: human PC-3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha SNAP/NAPA antigen affinity purified polyclonal antibody (#AAA127279) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Alpha SNAP/NAPA at approximately 33 kDa. The expected band size for Alpha SNAP/NAPA is at 33 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of Alpha SNAP/NAPA using anti-Alpha SNAP/NAPA antibody (AAA127279).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human 293T whole cell lysates,Lane 2: human MCF-7 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human K562 whole cell lysates,Lane 5: human THP-1 whole cell lysates,Lane 6: human PC-3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha SNAP/NAPA antigen affinity purified polyclonal antibody (#AAA127279) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Alpha SNAP/NAPA at approximately 33 kDa. The expected band size for Alpha SNAP/NAPA is at 33 kDa.)
Related Product Information for anti-AlphaSNAP/NAPA antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-AlphaSNAP/NAPA antibody
References
1. Andreeva, A. V., Kutuzov, M. A., Vaiskunaite, R., Profirovic, J., Meigs, T. E., Predescu, S., Malik, A. B., Voyno-Yasenetskaya, T. G-alpha-12 interaction with alpha-SNAP induces VE-cadherin localization at endothelial junctions and regulates barrier function. J. Biol. Chem. 280: 30376-30383, 2005. 2. Bronson, R. T., Lane, P. W. Hydrocephalus with hop gait (hyh): a new mutation on chromosome 7 in the mouse. Dev. Brain Res. 54: 131-136, 1990.3. Chae, T. H., Allen, K. M., Davisson, M. T., Sweet, H. O., Walsh, C. A. Mapping of the mouse hyh gene to a YAC/BAC contig on proximal chromosome 7. Mammalian Genome 13: 239-244, 2002.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,233 Da
NCBI Official Full Name
alpha-soluble NSF attachment protein
NCBI Official Synonym Full Names
N-ethylmaleimide-sensitive factor attachment protein, alpha
NCBI Official Symbol
NAPA
NCBI Official Synonym Symbols
SNAPA
NCBI Protein Information
alpha-soluble NSF attachment protein; alpha-SNAP
UniProt Protein Name
Alpha-soluble NSF attachment protein
UniProt Gene Name
NAPA
UniProt Synonym Gene Names
SNAPA; SNAP-alpha
UniProt Entry Name
SNAA_HUMAN
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Product Notes
The AlphaSNAP/NAPA napa (Catalog #AAA127279) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Alpha SNAP/NAPA Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha SNAP/NAPA can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the AlphaSNAP/NAPA napa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha SNAP/NAPA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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