IF (Immunofluorescence)
(Immunofluorescence staining of Alpha-Tubulin in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human Alpha-Tubulin polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Rabbit Alpha-Tubulin Polyclonal Antibody | anti-TUBA1A antibody
Alpha-Tubulin Antibody, Rabbit PAb, Antigen Affinity Purified
Gene Names
TUBA1A; LIS3; TUBA3; B-ALPHA-1
Reactivity
HumanPredicted to React with: Mouse, Rat, Rabbit, Rhesus, Pig, Chinese hamster, Bovine, Dog (Species predicted to react based on 100% sequence homology)
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
Alpha-Tubulin, Antibody; Alpha-Tubulin Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-B-ALPHA-1 Antibody; Anti-LIS3 Antibody; Anti-TUBA3 Antibody; anti-TUBA1A antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse, Rat, Rabbit, Rhesus, Pig, Chinese hamster, Bovine, Dog (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse, Rat, Rabbit, Rhesus, Pig, Chinese hamster, Bovine, Dog (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human TBA1A
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-TUBA1A antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human Alpha-Tubulin
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Alpha-Tubulin, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of Alpha-Tubulin in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human Alpha-Tubulin polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of Alpha-Tubulin in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human Alpha-Tubulin polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human Alpha-Tubulin in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human Alpha-Tubulin in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human Alpha-Tubulin in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human Alpha-Tubulin in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-TUBA1A antibody
TUBA1A is a highly-conserved gene with few changes among eukaryotes and few polymorphic variants in human populations. TUBA1A encodes the tubulin alpha-1A chain, a protein that is highly expressed in the cerebral cortex, hippocampus, cerebellum, and brainstem of the developing fetal brain, with a decrease in postnatal and adult stages. Alpha-and beta-tubulin subunits form dimers, which then co-assemble into microtubule polymers: dynamic, scaffold-like structures that perform key functions during neurogenesis, neuronal migration, and cortical organization. Microtubules are dynamic polymers that perform a range of mechanical tasks within the cell. Mutations in TUBA1A have been reported to cause a range of brain malformations.
References
Gardner JF, et al. (2018) Clinical and functional characterization of the recurrent tuba1a p.(arg2his) mutation. Brain Sci 8 (8): 145.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
46,297 Da
NCBI Official Full Name
Tubulin alpha-1A chain
NCBI Official Synonym Full Names
tubulin, alpha 1a
NCBI Official Symbol
TUBA1A
NCBI Official Synonym Symbols
LIS3; TUBA3; B-ALPHA-1
NCBI Protein Information
tubulin alpha-1A chain; hum-a-tub1; hum-a-tub2; tubulin B-alpha-1; tubulin alpha-3 chain; tubulin, alpha, brain-specific
UniProt Protein Name
Tubulin alpha-1A chain
UniProt Gene Name
TUBA1A
UniProt Synonym Gene Names
TUBA3
UniProt Entry Name
TBA1A_HUMAN
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Product Notes
The TUBA1A tuba1a (Catalog #AAA254514) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Alpha-Tubulin Antibody, Rabbit PAb, Antigen Affinity Purified reacts with Human Predicted to React with: Mouse, Rat, Rabbit, Rhesus, Pig, Chinese hamster, Bovine, Dog (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha-Tubulin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the TUBA1A tuba1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha-Tubulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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