Rabbit anti-Human, mouse ALPL Polyclonal Antibody | anti-ALPL antibody

ALPL Antibody (Center)

Cat. Num. AAA28664

• Gene Names: ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
• Reactivity: Human, mouse
• Applications: Flow Cytometry, ELISA, Immunohistochemistry, Western Blot, Immunofluorescence
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: ALPL, Antibody; ALPL Antibody (Center); Alkaline phosphatase; tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme; ALPL; anti-ALPL antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This ALPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the Central region of human ALPL.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 217-246
• Sequence Length: 524

• Applicable Applications for anti-ALPL antibody: Flow Cytometry (FC), ELISA (EIA), Immunohistochemistry Paraffin (IHC-P-Leica), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: IHC-P-Leica~~1:500; WB~~1:2000; IF~~1:10~50; FC~~1:10~50
• Function: Alkaline phosphatase that metabolizes various phosphate compounds and plays a key role in skeletal mineralization and adaptive thermogenesis (PubMed: 12162492, PubMed: 23688511, PubMed: 25982064). Has broad substrate specificity and can hydrolyze a considerable variety of compounds: however, only a few substrates, such as diphosphate (inorganic pyrophosphate; PPi), pyridoxal 5'-phosphate (PLP) and N- phosphocreatine are natural substrates (PubMed: 12162492, PubMed: 2220817). Plays an essential role in skeletal and dental mineralization via its ability to hydrolyze extracellular diphosphate, a potent mineralization inhibitor, to phosphate: it thereby promotes hydroxyapatite crystal formation and increases inorganic phosphate concentration (PubMed: 23688511, PubMed: 25982064). Acts in a nonredundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix (By similarity). Also promotes dephosphorylation of osteopontin (SSP1), an inhibitor of hydroxyapatite crystallization in its phosphorylated state; it is however unclear whether ALPL/TNAP mediates SSP1 dephosphorylation via a direct or indirect manner (By similarity). Catalyzes dephosphorylation of PLP to pyridoxal (PL), the transportable form of vitamin B6, in order to provide a sufficient amount of PLP in the brain, an essential cofactor for enzymes catalyzing the synthesis of diverse neurotransmitters (PubMed: 2220817, PubMed: 20049532). Additionally, also able to mediate ATP degradation in a stepwise manner to adenosine, thereby regulating the availability of ligands for purinergic receptors (By similarity). Also capable of dephosphorylating microbial products, such as lipopolysaccharides (LPS) as well as other phosphorylated small-molecules, such as poly-inosine:cytosine (poly I:C) (PubMed:28448526). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating hydrolysis of N-phosphocreatine to initiate a futile cycle of creatine dephosphorylation and phosphorylation (By similarity). During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work (By similarity).
• Cellular Location: Cell membrane; Lipid-anchor, GPI-anchor Extracellular vesicle membrane {ECO:0000250|UniProtKB:P09242}; Lipid- anchor, GPI-anchor {ECO:0000250|UniProtKB:P09242}. Mitochondrion membrane {ECO:0000250|UniProtKB:P09242}; Lipid-anchor, GPI-anchor {ECO:0000250|UniProtKB:P09242}. Mitochondrion intermembrane space {ECO:0000250|UniProtKB:P09242}. Note=Localizes to special class of extracellular vesicles, named matrix vesicles (MVs), which are released by osteogenic cells. Localizes to the mitochondria of thermogenic fat cells: tethered to mitochondrial membranes via a GPI-anchor and probably resides in the mitochondrion intermembrane space {ECO:0000250|UniProtKB:P09242}.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM (Flow Cytometry)

(ALPL Antibody (Center) (Cat. AAA28664) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of ALPL Antibody (Center)(Cat# AAA28664) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

WB (Western Blot)

(All lanes : Anti-ALPL Antibody (Center) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: F9 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-ALPL Antibody (Center) at 1:2000 dilution + F9 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using AAA28664 performed on the Leica® BOND RXm.Samples were incubated with primary antibody(1/500) for 1 hours at room temperature.A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis ofparaffin-embedded human kidney tissue using AAA28664 performed on the Leica® BOND RXm.Samples were incubated with primary antibody(1/500) for 1 hours at room temperature.A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

Related Product Information for anti-ALPL antibody

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The genes for the first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. This protein is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms.

Product Categories/Family for anti-ALPL antibody

Cancer; Cell Biology; Immunology

References

Panuccio,V., Am. J. Kidney Dis. 50 (6), 1001-1008 (2007)
Brun-Heath,I., Eur J Med Genet 50 (5), 367-378 (2007)
So,P.P., J. Rheumatol. 34 (6), 1313-1322 (2007)

NCBI and Uniprot Product Information

• NCBI GI #: 116734717
• NCBI GeneID: 249
• NCBI Accession #: NP_000469.3; NP_001120973.2; NP_001170991.1
• NCBI GenBank Nucleotide #: NM_000478.4
• UniProt Accession #: P05186; O75090; Q2TAI7; Q59EJ7; Q5BKZ5; Q5VTG5; Q6NZI8; Q8WU32; A1A4E7; B2RMP8; B7Z387; B7Z4Y6
• NCBI Official Full Name: alkaline phosphatase, tissue-nonspecific isozyme isoform 1
• NCBI Official Synonym Full Names: alkaline phosphatase, liver/bone/kidney
• NCBI Official Symbol: ALPL
• NCBI Official Synonym Symbols: HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
• NCBI Protein Information: alkaline phosphatase, tissue-nonspecific isozyme
• UniProt Protein Name: Alkaline phosphatase, tissue-nonspecific isozyme
• UniProt Gene Name: ALPL
• UniProt Synonym Gene Names: AP-TNAP; TNSALP
• UniProt Entry Name: PPBT_HUMAN