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IF (Immunofluorescence)
(Confocal immunofluorescent analysis of AMACR Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human AMACR Polyclonal Antibody | anti-AMACR antibody
AMACR Antibody (C-term)
Gene Names
AMACR; RM; RACE; CBAS4; AMACRD
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
AMACR, Antibody; AMACR Antibody (C-term); Alpha-methylacyl-CoA racemase; 2-methylacyl-CoA racemase; AMACR; anti-AMACR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This AMACR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-351 amino acids from the C-terminal region of human AMACR.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
323-351
Sequence Length
394
Applicable Applications for anti-AMACR antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of AMACR Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of AMACR Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
FCM/FACS (Flow Cytometry)
(AMACR Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(AMACR Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(AMACR antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AMACR antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(AMACR antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AMACR antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(AMACR Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the AMACR antibody detected the AMACR protein (arrow).)
WB (Western Blot)
(AMACR Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the AMACR antibody detected the AMACR protein (arrow).)
Related Product Information for anti-AMACR antibody
This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described.
Product Categories/Family for anti-AMACR antibody
References
Murray, N.P., et al. Oncol. Rep. 24(3):687-692(2010) Sonwalkar, S.A., et al. Histopathology 56(7):900-907(2010) Lakis, S., et al. World J. Gastroenterol. 16(20):2476-2483(2010) Chen, W., et al. Mol. Biol. Rep. 36(3):423-430(2009) Mubiru, J.N., et al. Gene 327(1):89-98(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42387
NCBI Official Full Name
alpha-methylacyl-CoA racemase isoform 3
NCBI Official Synonym Full Names
alpha-methylacyl-CoA racemase
NCBI Official Symbol
AMACR
NCBI Official Synonym Symbols
RM; RACE; CBAS4; AMACRD
NCBI Protein Information
alpha-methylacyl-CoA racemase
UniProt Protein Name
Alpha-methylacyl-CoA racemase
UniProt Gene Name
AMACR
UniProt Entry Name
AMACR_HUMAN
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Product Notes
The AMACR amacr (Catalog #AAA288758) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 323-351. The AMACR Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AMACR can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the AMACR amacr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMACR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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