IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with AMBRA1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit AMBRA1 Polyclonal Antibody | anti-AMBRA1 antibody
AMBRA1 Antibody (N-term)
Gene Names
AMBRA1; DCAF3; WDR94
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
AMBRA1, Antibody; AMBRA1 Antibody (N-term); Activating molecule in BECN1-regulated autophagy protein 1; AMBRA1; KIAA1736; anti-AMBRA1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This AMBRA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-55 amino acids from the N-terminal region of human AMBRA1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
27-55
Sequence Length
761
Applicable Applications for anti-AMBRA1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with AMBRA1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with AMBRA1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of AMBRA1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). AMBRA1(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of AMBRA1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). AMBRA1(arrow) was detected using the purified Pab.)
Related Product Information for anti-AMBRA1 antibody
AMBRA1 regulates autophagy and development of the nervous system. This protein is involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation.
Product Categories/Family for anti-AMBRA1 antibody
References
Maria Fimia G.,Nature 447:1121-1125(2007).
Nagase T.,DNA Res. 7:347-355(2000).
Ota T.,Nat. Genet. 36:40-45(2004).
Nagase T.,DNA Res. 7:347-355(2000).
Ota T.,Nat. Genet. 36:40-45(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
142507
NCBI Official Full Name
activating molecule in BECN1-regulated autophagy protein 1 isoform 1
NCBI Official Synonym Full Names
autophagy/beclin-1 regulator 1
NCBI Official Symbol
AMBRA1
NCBI Official Synonym Symbols
DCAF3; WDR94
NCBI Protein Information
activating molecule in BECN1-regulated autophagy protein 1
UniProt Protein Name
Activating molecule in BECN1-regulated autophagy protein 1
UniProt Gene Name
AMBRA1
UniProt Synonym Gene Names
KIAA1736
UniProt Entry Name
AMRA1_HUMAN
Similar Products
Product Notes
The AMBRA1 ambra1 (Catalog #AAA288865) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 27-55. The AMBRA1 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's AMBRA1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the AMBRA1 ambra1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMBRA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.