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IF (Immunofluorescence)
(Immunofluorescence staining of A549 cells with AAA232934 at 1:266,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Rabbit anti-Human, Mouse AMIGO2 Polyclonal Antibody | anti-AMIGO2 antibody
AMIGO2 Antibody
Gene Names
AMIGO2; ALI1; DEGA; AMIGO-2
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
AMIGO2, Antibody; AMIGO2 Antibody; Amphoterin-induced protein 2; AMIGO-2; Alivin-1; Differentially expressed in gastric adenocarcinomas; DEGA; ALI1; AMIGO2; anti-AMIGO2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
522
Applicable Applications for anti-AMIGO2 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human Amphoterin-induced protein 2 protein (420-522AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-46759 / sc-46761 / sc-46760
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescence staining of A549 cells with AAA232934 at 1:266,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of A549 cells with AAA232934 at 1:266,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
IHC (Immunohistochemisry)
(IHC image of AAA232934 diluted at 1:800 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemisry)
(IHC image of AAA232934 diluted at 1:800 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohiostchemistry)
(IHC image of AAA232934 diluted at 1:800 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohiostchemistry)
(IHC image of AAA232934 diluted at 1:800 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot)
(Western BlotPositive WB detected in: Rat liver tissueAll lanes: AMIGO2 antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 58 KDaObserved band size: 58 KDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Rat liver tissueAll lanes: AMIGO2 antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 58 KDaObserved band size: 58 KDa)
Related Product Information for anti-AMIGO2 antibody
Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas.
References
[1]"DEGA/AMIGO-2, a leucine-rich repeat family member, differentially expressed in human gastric adenocarcinoma: effects on ploidy, chromosomal stability, cell adhesion/migration and tumorigenicity."Rabenau K.E., O'Toole J.M., Bassi R., Kotanides H., Witte L., Ludwig D.L., Pereira D.S.Oncogene 23: 5056-5067(2004). [2]"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8: 399-399(2007). [3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGenome Res. 14: 2121-2127(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,934 Da
NCBI Official Full Name
amphoterin-induced protein 2
NCBI Official Synonym Full Names
adhesion molecule with Ig like domain 2
NCBI Official Symbol
AMIGO2
NCBI Official Synonym Symbols
ALI1; DEGA; AMIGO-2
NCBI Protein Information
amphoterin-induced protein 2
UniProt Protein Name
Amphoterin-induced protein 2
UniProt Gene Name
AMIGO2
UniProt Synonym Gene Names
DEGA
UniProt Entry Name
AMGO2_HUMAN
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Product Notes
The AMIGO2 amigo2 (Catalog #AAA232934) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AMIGO2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AMIGO2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the AMIGO2 amigo2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMIGO2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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