Rabbit AMPK alpha Polyclonal Antibody | anti-PRKAA1 antibody

AMPK alpha Antibody

Cat. Num. AAA322404

• Gene Names: PRKAA1; AMPK; AMPKa1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Peptide affinity purification
• Synonyms: AMPK alpha, Antibody; AMPK alpha Antibody; 5 AMP activated protein kinase alpha 1catalytic subunit; 5 AMP activated protein kinase catalytic alpha 1 chain; 5' AMP activated protein kinase catalytic subunit alpha 1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AAPK1; AAPK1_HUMAN; ACACA kinase; acetyl CoA carboxylase kinase; AI194361; AI450832; AL024255; AMP-activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK 1; AMPK alpha 1; AMPK alpha 1 chain; AMPK; AMPK subunit alpha-1; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; cb116; EC 2.7.11.1; HMG CoA reductase kinase; HMGCR kinase; hormone sensitive lipase kinase; Hydroxymethylglutaryl CoA reductase kinase; im:7154392; kinase AMPK alpha1; MGC33776; MGC57364; OTTHUMP00000161795; OTTHUMP00000161796; PRKAA 1; PRKAA1; Protein kinase AMP activated alpha 1 catalytic subunit; SNF1-like protein AMPK; SNF1A; Tau protein kinase PRKAA1; wu:fa94c10; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AAPK2_HUMAN; Acetyl-CoA carboxylase kinase; AMPK alpha 2 chain; AMPK subunit alpha-2; AMPK2; AMPKa2; AMPKalpha2; Hydroxymethylglutaryl-CoA reductase kinase; PRKAA; PRKAA2; Protein kinase AMP activated alpha 2 catalytic subunit; Protein kinase AMP activated catalytic subunit alpha 2; anti-PRKAA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: AMPK alpha Antibody detects endogenous levels of total AMPK alpha.
• Purity/Purification: Peptide affinity purification
• Form/Format: IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PRKAA1 antibody: ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human AMPK alpha, corresponding to a region within the internal amino acids.
• Preparation and Storage: Store at -20°C.

WB (Western Blot)

(Western blot analysis of AMPK alpha expression in Adriamycin treated COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence)

(AAA322404 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry)

(AAA322404 at 1/100 staining Rat spleen tissue by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigenretrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°Covernight. An HRP conjugated anti-Rabbit antibody was usedas the secondary antibody.)

Related Product Information for anti-PRKAA1 antibody

AMPKA2 a protein kinase of the CAMKL family. The holoenzyme consists of a catalytic subunit (alpha) and two regulatory subunits (beta, gamma).

References

Li M, Li J, Zeng R, Yang J, Liu J, Zhang Z, Song X, Yao Z, Ma C, Li W, Wang K, Wei L; Journal: J Virol. Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis. Li ZH, Xiong QY, Xu L, Duan P, Yang QO, Zhou P, Tu JH; Journal: Oncotarget. miR-29a regulated ER-positive breast cancer cell growth and invasion and is involved in the insulin signaling pathway. Xie X, Huang X, Tang H, Ye F, Yang L, Guo X, Tian Z, Xie X, Peng C, Xie X; Journal: Curr Cancer Drug Targets. Diallyl Disulfide Inhibits Breast Cancer Stem Cell Progression and Glucose Metabolism by Targeting CD44/PKM2/AMPK Signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 94557301
• NCBI GeneID: 5562
• NCBI Accession #: NP_006242.5
• NCBI GenBank Nucleotide #: NM_006251.5
• UniProt Accession #: Q13131; O00286; Q5D0E1; Q86VS1; Q9UNQ4; A8MTQ6; B2R7E1
• Molecular Weight: 62kDa
• NCBI Official Full Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1
• NCBI Official Synonym Full Names: protein kinase AMP-activated catalytic subunit alpha 1
• NCBI Official Symbol: PRKAA1
• NCBI Official Synonym Symbols: AMPK; AMPKa1
• NCBI Protein Information: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• UniProt Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• UniProt Gene Name: PRKAA1
• UniProt Synonym Gene Names: AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase