WB (Western Blot)
(Detection of human Androgen Receptor by western blot. Samples: Whole cell lysate (50 ug) from LNCaP and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Androgen Receptor antibody (AAA212413 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human Androgen Receptor Polyclonal Antibody | anti-AR antibody
Rabbit anti-Androgen Receptor Antibody, Affinity Purified
Gene Names
AR; KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Androgen Receptor, Antibody; Rabbit anti-Androgen Receptor Antibody, Affinity Purified; HYSP1; NR3C4; TFM; SMAX1; nuclear receptor subfamily 3 group C member 4; KD; dihydrotestosterone receptor; AIS; DHTR; HUMARA; AR8; androgen receptor; SBMA; Androgen receptor; anti-AR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-AR antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat, Dog, Pig, Monkey, Chimpanzee
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Androgen Receptor by western blot. Samples: Whole cell lysate (50 ug) from LNCaP and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Androgen Receptor antibody (AAA212413 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human Androgen Receptor by western blot. Samples: Whole cell lysate (50 ug) from LNCaP and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Androgen Receptor antibody (AAA212413 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human Androgen Receptor by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from LNCaP cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Androgen Receptor antibody (AAA212413 lot 2) used for IP at 6 ug per reaction. Androgen Receptor was also immunoprecipitated by a previous lot of this antibody (AAA212413 lot 1) and a second antibody against a different epitiope of Androgen Receptor . For blotting immunoprecipitated Androgen Receptor, AAA212413 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human Androgen Receptor by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from LNCaP cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Androgen Receptor antibody (AAA212413 lot 2) used for IP at 6 ug per reaction. Androgen Receptor was also immunoprecipitated by a previous lot of this antibody (AAA212413 lot 1) and a second antibody against a different epitiope of Androgen Receptor . For blotting immunoprecipitated Androgen Receptor, AAA212413 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human Androgen Receptor by immunohistochemistry. Sample: FFPE section of human testiclar seminoma. Antibody: Affinity purified rabbit anti-Androgen Receptor (AAA212413 Lot 2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Androgen Receptor by immunohistochemistry. Sample: FFPE section of human testiclar seminoma. Antibody: Affinity purified rabbit anti-Androgen Receptor (AAA212413 Lot 2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-AR antibody
The androgen receptor (AR) functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes [taken from NCBI Entrez Gene (Gene ID: 367)].
Product Categories/Family for anti-AR antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
98,989 Da
NCBI Official Full Name
androgen receptor isoform 1
NCBI Official Synonym Full Names
androgen receptor
NCBI Official Symbol
AR
NCBI Official Synonym Symbols
KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
NCBI Protein Information
androgen receptor; dihydrotestosterone receptor; androgen nuclear receptor variant 2; nuclear receptor subfamily 3 group C member 4
UniProt Protein Name
Androgen receptor
UniProt Gene Name
AR
UniProt Synonym Gene Names
DHTR; NR3C4
UniProt Entry Name
ANDR_HUMAN
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Product Notes
The AR ar (Catalog #AAA212413) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Androgen Receptor Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Androgen Receptor can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the AR ar for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Androgen Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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