IHC (Immunohiostchemistry)
(Angiotensinogen was detected in paraffin-embedded sections of mouse kidney tissues using rabbit anti- Angiotensinogen Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
Rabbit anti-Mouse Angiotensinogen Polyclonal Antibody | anti-AGT antibody
Anti-Angiotensinogen Antibody
Gene Names
Agt; AngI; AngII; Aogen; AI265500; Serpina8
Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Angiotensinogen, Antibody; Anti-Angiotensinogen Antibody; AGT; Angiotensin I; Angiotensin II; Angiotensin III; Angiotensin-1; Angiotensin-2; Angiotensin-3; Angiotensinogen; ANGT; ANHU; P01019; Pre angiotensinogen; Serpin A8; SERPINA8; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); anti-AGT antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
477
Applicable Applications for anti-AGT antibody
IHC (Immunohistochemistry), WB (Western Blot)
Notes
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E Coli-derived mouse Angiotensinogen recombinant protein (Position: D25-A115). Mouse Angiotensinogen shares 62.2% and 87.9% amino acid (aa) sequence identity with human and rat Angiotensinogen, respectively.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Angiotensinogen was detected in paraffin-embedded sections of mouse kidney tissues using rabbit anti- Angiotensinogen Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohiostchemistry)
(Angiotensinogen was detected in paraffin-embedded sections of mouse kidney tissues using rabbit anti- Angiotensinogen Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
WB (Western Blot)
(Western blot analysis of Angiotensinogen expression in mouse liver extract (lane 1), mouse spleen extract (lane 2), mouse testis extract (lane 3) and mouse kidney extract (lane 4). Angiotensinogen at 56KD was detected using rabbit anti- Angiotensinogen Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)
WB (Western Blot)
(Western blot analysis of Angiotensinogen expression in mouse liver extract (lane 1), mouse spleen extract (lane 2), mouse testis extract (lane 3) and mouse kidney extract (lane 4). Angiotensinogen at 56KD was detected using rabbit anti- Angiotensinogen Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)
Related Product Information for anti-AGT antibody
Rabbit IgG polyclonal antibody for Angiotensinogen(AGT) detection.
Background: AGT(ANGIOTENSINOGEN), also called SERPINA8, is an alpha-2-globulin that is produced constitutively and released into the circulation mainly by the liver. ATG is a member of the serpin family, although it is not known to inhibit other enzymes, unlike most serpins. Angiotensinogen is also known as renin substrate. The AGT gene is mapped on 1q42.2. And the human angiotensinogen gene contains 5 exons. The expression of AGT and enzymes required for its conversion to angiotensin II in human adipose tissue. Mutations in this AGT gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.
Background: AGT(ANGIOTENSINOGEN), also called SERPINA8, is an alpha-2-globulin that is produced constitutively and released into the circulation mainly by the liver. ATG is a member of the serpin family, although it is not known to inhibit other enzymes, unlike most serpins. Angiotensinogen is also known as renin substrate. The AGT gene is mapped on 1q42.2. And the human angiotensinogen gene contains 5 exons. The expression of AGT and enzymes required for its conversion to angiotensin II in human adipose tissue. Mutations in this AGT gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.
References
1. Abonia, J. P., Abel, K. J., Eddy, R. L., Elliott, R. W., Chapman, V. M., Shows, T. B., Gross, K. W. Linkage of Agt and Actsk-1 to distal mouse chromosome 8 loci: a new conserved linkage. Mammalian Genome 4: 25-32, 1993.
2. Arakawa, K., Minohara, A., Yamada, J., Nakamura, M. Enzymatic degradation and electrophoresis of human angiotensin I. Biochim. Biophys. Acta 168: 106-112, 1968.
3. Basso N, Terragno NA (December 2001). "History about the discovery of the renin-angiotensin system". Hypertension 38 (6): 1246-9.
2. Arakawa, K., Minohara, A., Yamada, J., Nakamura, M. Enzymatic degradation and electrophoresis of human angiotensin I. Biochim. Biophys. Acta 168: 106-112, 1968.
3. Basso N, Terragno NA (December 2001). "History about the discovery of the renin-angiotensin system". Hypertension 38 (6): 1246-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
51,990 Da
NCBI Official Full Name
Angiotensinogen
NCBI Official Synonym Full Names
angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
NCBI Official Symbol
Agt
NCBI Official Synonym Symbols
AngI; AngII; Aogen; AI265500; Serpina8
NCBI Protein Information
angiotensinogen
UniProt Protein Name
Angiotensinogen
UniProt Gene Name
Agt
UniProt Synonym Gene Names
Serpina8; Ang I; Ang II; Ang III; Ang IV
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Product Notes
The AGT agt (Catalog #AAA46738) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Angiotensinogen Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Angiotensinogen can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the AGT agt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Angiotensinogen, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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