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WB (Western Blot)
(Rabbit anti-Ang-1 was used at a 1:500 dilution to detect mouse Ang-1 by western blot against supernatants of mouse angiopoietin-expressing endothelial cells. Lane 1 - wt endothelial cells. Lane 2 - mouse Ang-1 (clone 1-8) expressing cells. Lane 3 - mouse Ang-1 (clone 1-15) expressing cells. Lane 4 - mouse Ang-2 (clone 2-9) expressing cells.)
Rabbit anti-Human, Mouse ANGPT1 / Angiopoietin-1 Polyclonal Antibody | anti-ANGPT1 antibody
Rabbit Polyclonal to Mouse ANGPT1 / Angiopoietin-1
Gene Names
ANGPT1; AGP1; AGPT; ANG1
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Sterile filtered
Synonyms
ANGPT1 / Angiopoietin-1, Antibody; Rabbit Polyclonal to Mouse ANGPT1 / Angiopoietin-1; Anti-ANGPT1 / Angiopoietin-1 Antibody (N-Terminus) IHC-plus; Angiopoietin 1; Angiopoietin-1; AGPT; AGP1; ANG-1; ANG1; KIAA0003; Mouse ANGPT1; anti-ANGPT1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Synthetic peptide N-Q-R-R-N-P-E-N-G-G-R-R-Y-N-R-I-Q-H-G-Q corresponding to a region (aa 21-40) near the N-terminus of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Purity/Purification
Sterile filtered
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
80 mg/ml (varies by lot)
Sequence Length
498
Applicable Applications for anti-ANGPT1 antibody
ELISA, WB (Western Blot), IHC (Immunohistochemistry)
Target Species
Mouse
Immunogen Description
Synthetic peptide from mouse ANGPT1 / Angiopoietin-1.
Immunogen Type
Synthetic peptide
Immunogen
ANGPT1 / Angiopoietin-1 antibody was raised against synthetic peptide from mouse ANGPT1 / Angiopoietin-1.
Antigen Modification
N-Terminus
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
WB (Western Blot)
(Rabbit anti-Ang-1 was used at a 1:500 dilution to detect mouse Ang-1 by western blot against supernatants of mouse angiopoietin-expressing endothelial cells. Lane 1 - wt endothelial cells. Lane 2 - mouse Ang-1 (clone 1-8) expressing cells. Lane 3 - mouse Ang-1 (clone 1-15) expressing cells. Lane 4 - mouse Ang-2 (clone 2-9) expressing cells.)
WB (Western Blot)
(Rabbit anti-Ang-1 was used at a 1:500 dilution to detect mouse Ang-1 by western blot against supernatants of mouse angiopoietin-expressing endothelial cells. Lane 1 - wt endothelial cells. Lane 2 - mouse Ang-1 (clone 1-8) expressing cells. Lane 3 - mouse Ang-1 (clone 1-15) expressing cells. Lane 4 - mouse Ang-2 (clone 2-9) expressing cells.)
IHC (Immunohistochemisry)
(Anti-ANGPT1 / Angiopoietin-1 antibody IHC staining of human lung, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
IHC (Immunohistochemisry)
(Anti-ANGPT1 / Angiopoietin-1 antibody IHC staining of human lung, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
IHC (Immunohiostchemistry)
(Anti-ANGPT1 / Angiopoietin-1 antibody IHC staining of mouse liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
IHC (Immunohiostchemistry)
(Anti-ANGPT1 / Angiopoietin-1 antibody IHC staining of mouse liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
IHC (Immunohistochemistry)
(Anti-ANGPT1 / Angiopoietin-1 antibody IHC staining of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
IHC (Immunohistochemistry)
(Anti-ANGPT1 / Angiopoietin-1 antibody IHC staining of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,456 Da
NCBI Official Full Name
angiopoietin-1 isoform 1
NCBI Official Synonym Full Names
angiopoietin 1
NCBI Official Symbol
ANGPT1
NCBI Official Synonym Symbols
AGP1; AGPT; ANG1
NCBI Protein Information
angiopoietin-1; ANG-1
UniProt Protein Name
Angiopoietin-1
UniProt Gene Name
ANGPT1
UniProt Synonym Gene Names
KIAA0003; ANG-1
UniProt Entry Name
ANGP1_HUMAN
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Product Notes
The ANGPT1 angpt1 (Catalog #AAA50697) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Mouse ANGPT1 / Angiopoietin-1 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ANGPT1 / Angiopoietin-1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ANGPT1 angpt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANGPT1 / Angiopoietin-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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