Rabbit anti-Human Annexin V/ANXA5 Polyclonal Antibody | anti-ANXA5 antibody

Anti-Annexin V/ANXA5 Antibody, Rabbit Polyclonal

Cat. Num. AAA254934

• Gene Names: ANXA5; PP4; ANX5; ENX2; RPRGL3; HEL-S-7
• Reactivity: Human
• Applications: Immunoprecipitation, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Annexin V/ANXA5, Antibody; Anti-Annexin V/ANXA5 Antibody, Rabbit Polyclonal; ANXA5 Antibody, Rabbit PAb, Antigen Affinity Purified; annexin A5; Anti-ANX5 Antibody; Anti-ENX2 Antibody; Anti-HEL-S-7 Antibody; Anti-PP4 Antibody; Anti-RPRGL3 Antibody; anti-ANXA5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Annexin V/ANXA5
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ANXA5 antibody: IP (Immunoprecipitation), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human ANXA5 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human ANXA5 (rh ANXA5). ANXA5 specific IgG was purified by Human ANXA5 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-ANXA5 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:35 kDa)

IP (Immunoprecipitation)

(ANXA5 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-ANXA5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ANXA5 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa)

Related Product Information for anti-ANXA5 antibody

The placental anticoagulant protein Annexin A5 (ANXA5) is a multifunctional protein that is highly expressed on the apical surfaces of syncytiotrophoblasts, and plays an important role in haemostatic regulations, maintaining blood fluidity of the placenta. Annexin A5 (ANXA5) is a protein abundantly expressed in normal placenta where it contributes to the healthy outcome of a pregnancy. Lower ANXA5 levels have been observed in M2/ANXA5 haplotype carrying chorion. The association found between the maternal carriage of the M2/ANXA5 haplotype and an elevated risk of IUGR and/or PE supports the hypothesis that carrier status of this haplotype and the consequently reduced placental ANXA5 expression might be responsible, at least partially, for the onset of these gestational vascular complications. ANXA5 could be used as a biomarker for the early detection of PE and for the prediction of its severity. ANXA5 as an embryonic anticoagulant that appears deficient in contiguous specter of thrombophilia-related pregnancy complications culminating more frequently in miscarriage in a maternal M2 carrier background. As a potential indicator for malignancy and lymphatic metastasis, ANXA5 overexpression increases in vitro migration and invasion of Hca-P cell, promotes in vivo malignancy, LNM rate and level of Hca-P-transplanted mice. Hereditary thrombophilias can impair vascular placental functions and predispose to the birth of small-for-gestational age (SGA) babies. The placental anticoagulant protein annexin A5 (ANXA5) may contribute to this process. A functional haplotype (M2) within the ANXA5 gene is associated with fetal loss and venous thrombosis.

References

Cederholm A, et al. (2007) Annexin A5 as a novel player in prevention of atherothrombosis in SLE and in the general population. Ann N Y Acad Sci. 1108: 96-103.Schlaepfer DD, et al. (1992) Inhibition of Protein Kinase C by AnnexinI¤. Biochemistry. 31: 1886-91.Vermes I, et al. (1995) A novel assay for apoptosis-flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin I¤. J Immunol Methods. 184 (1): 39-51.

NCBI and Uniprot Product Information

• NCBI GI #: 4502107
• NCBI GeneID: 308
• NCBI Accession #: NP_001145.1
• NCBI GenBank Nucleotide #: NM_001154.3
• UniProt Accession #: P08758; Q6FHB3; Q6FI16; Q8WV69; Q9UDH9; D3DNW7
• Molecular Weight: 35,937 Da
• NCBI Official Full Name: annexin A5
• NCBI Official Synonym Full Names: annexin A5
• NCBI Official Symbol: ANXA5
• NCBI Official Synonym Symbols: PP4; ANX5; ENX2; RPRGL3; HEL-S-7
• NCBI Protein Information: annexin A5; CBP-I; PAP-I; VAC-alpha; annexin V; annexin-5; anchorin CII; endonexin II; lipocortin V; calphobindin I; thromboplastin inhibitor; vascular anticoagulant-alpha; epididymis secretory protein Li 7; placental anticoagulant protein 4; placental an
• UniProt Protein Name: Annexin A5
• UniProt Gene Name: ANXA5
• UniProt Synonym Gene Names: ANX5; ENX2; PP4; CBP-I; PP4; PAP-I; VAC-alpha
• UniProt Entry Name: ANXA5_HUMAN