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WB (Western Blot)
(Anti-ANXA6 rabbit polyclonal antibody at 1:1000 dilutionLane A: NIH-3T3 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: NCI-H1299 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:76 kDaObserved band size:65 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human Annexin VI/ANXA6 Polyclonal Antibody | anti-ANXA6 antibody
Anti-Annexin VI/ANXA6 Antibody, Rabbit Polyclonal
Gene Names
ANXA6; ANX6; CBP68
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Annexin VI/ANXA6, Antibody; Anti-Annexin VI/ANXA6 Antibody, Rabbit Polyclonal; Annexin VI/ANXA6 Antibody, Rabbit PAb, Antigen Affinity Purified; annexin A6; Anti-ANX6 Antibody; Anti-CBP68 Antibody; anti-ANXA6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Annexin VI/ANXA6
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ANXA6 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human Annexin VI/ANXA6 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human Annexin VI/ANXA6 (rh Annexin VI/ANXA6). Annexin VI/ANXA6 specific IgG was purified by Human Annexin VI/ANXA6 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-ANXA6 rabbit polyclonal antibody at 1:1000 dilutionLane A: NIH-3T3 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: NCI-H1299 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:76 kDaObserved band size:65 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-ANXA6 rabbit polyclonal antibody at 1:1000 dilutionLane A: NIH-3T3 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: NCI-H1299 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:76 kDaObserved band size:65 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(ANXA6 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg NIH-3T3 Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate2 uL anti-ANXA6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ANXA6 rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 76 kDaObserved band size: 76 kDa)
IP (Immunoprecipitation)
(ANXA6 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg NIH-3T3 Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate2 uL anti-ANXA6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ANXA6 rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 76 kDaObserved band size: 76 kDa)
IHC (Immunohistochemisry)
(Immunochemical staining of human ANXA6 in human testis with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemisry)
(Immunochemical staining of human ANXA6 in human testis with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human ANXA6 in human pancreas with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human ANXA6 in human pancreas with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human ANXA6 in human liver with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human ANXA6 in human liver with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-ANXA6 antibody
Annexin A6, also known as ANXA6 or ANXAI¥, belongs to a family of Ca2+-dependent membrane and phospholipid binding proteins. Members of this family have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 6 is phosphorylated in vivo associated with cell growth. Annexin 6 was not phosphorylated in quiescent cells, but was phosphorylated on serine and to a lesser extent threonine, several hours following cell stimulation. Experiment has revealed the presence of annexin A6 on the cell surface of variety cells as putative receptors and/or binding proteins for chondroitin sulfate proteoglycans, helping cells to bind with this extracellular matrix glycosaminoglycan chondroitin sulfate which is related to the cell-substratum adhesion. A post-tranlational modification other than direct protein phosphorylation may influence the activity of annexin6 and provide evidence linking cell growth with regulation of annexin 6 function.
References
Takagi H, et al. (2002) Annexin 6 is a putative cell surface receptor for chondroitin sulfate chains. J Cell Sci. 115 (16): 3309-18.Moss SE, et al. (1992) A growth-dependent post-translational modification of annexin VI. Biochim Biophys Acta. 1160 (1): 120-6.Song G, et al. (1998) Altered cardiac annexin mRNA and protein levels in the left ventricle of patients with end-stage heart failure. J Mol Cell Cardio. 30 (3): 443-51.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75,873 Da
NCBI Official Full Name
annexin A6 isoform 1
NCBI Official Synonym Full Names
annexin A6
NCBI Official Symbol
ANXA6
NCBI Official Synonym Symbols
ANX6; CBP68
NCBI Protein Information
annexin A6; p68; p70; CPB-II; annexin-6; calelectrin; lipocortin VI; calphobindin II; calphobindin-II; chromobindin-20; annexin VI (p68); 67 kDa calelectrin; calcium-binding protein p68
UniProt Protein Name
Annexin A6
UniProt Gene Name
ANXA6
UniProt Synonym Gene Names
ANX6; CPB-II
UniProt Entry Name
ANXA6_HUMAN
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Product Notes
The ANXA6 anxa6 (Catalog #AAA255334) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Annexin VI/ANXA6 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Annexin VI/ANXA6 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ANXA6 anxa6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin VI/ANXA6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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