WB (Western Blot)
(Western blot analysis of lysates from SH-SY5Y,U-937 cell line and mouse brain tissue lysate(from left to right), using ANP32E Antibody(N-term) (AAA289895). AP20559a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was.)
Rabbit ANP32E Polyclonal Antibody | anti-ANP32E antibody
ANP32E Antibody (N-term)
Gene Names
ANP32E; LANPL; LANP-L
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
ANP32E, Antibody; ANP32E Antibody (N-term); Acidic leucine-rich nuclear phosphoprotein 32 family member E; LANP-like protein; LANP-L; ANP32E; anti-ANP32E antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ANP32E antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 21-44 amino acids from the N-terminal region of human ANP32E.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
220
Applicable Applications for anti-ANP32E antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Function
Histone chaperone that specifically mediates the genome- wide removal of histone H2A.Z/H2AFZ from the nucleosome: removes H2A.Z/H2AFZ from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AFZ in the nucleosome. May stabilize the evicted H2A.Z/H2AFZ-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511). Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis.
Cellular location
Cytoplasm. Nucleus
Tissue location
Expressed in peripheral blood leukocytes, colon, small intestine, prostate, thymus, spleen, skeletal muscle, liver and kidney.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from SH-SY5Y,U-937 cell line and mouse brain tissue lysate(from left to right), using ANP32E Antibody(N-term) (AAA289895). AP20559a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was.)
WB (Western Blot)
(Western blot analysis of lysates from SH-SY5Y,U-937 cell line and mouse brain tissue lysate(from left to right), using ANP32E Antibody(N-term) (AAA289895). AP20559a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was.)
IF (Immunofluorescence)
(Fluorescent image of SH-SY5Y cells stained with ANP32E Antibody (N-term). AAA289895 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of SH-SY5Y cells stained with ANP32E Antibody (N-term). AAA289895 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Related Product Information for anti-ANP32E antibody
Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis process by modulating PP2A activity (By similarity).
Product Categories/Family for anti-ANP32E antibody
References
Jiang M.,et al.Cytogenet. Genome Res. 97:68-71(2002).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30692
NCBI Official Full Name
acidic leucine-rich nuclear phosphoprotein 32 family member E isoform 2
NCBI Official Synonym Full Names
acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
NCBI Official Symbol
ANP32E
NCBI Official Synonym Symbols
LANPL; LANP-L
NCBI Protein Information
acidic leucine-rich nuclear phosphoprotein 32 family member E
UniProt Protein Name
Acidic leucine-rich nuclear phosphoprotein 32 family member E
UniProt Gene Name
ANP32E
UniProt Synonym Gene Names
LANP-L
UniProt Entry Name
AN32E_HUMAN
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Product Notes
The ANP32E anp32e (Catalog #AAA289895) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ANP32E Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ANP32E can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ANP32E anp32e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANP32E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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