WB (Western Blot)
(Western blot analysis of extracts of HEK-293 cells, using Antizyme inhibitor 1 antibody.)
Rabbit Antizyme Inhibitor 1 Polyclonal Antibody | anti-AZIN1 antibody
Antizyme Inhibitor 1 Antibody
Gene Names
AZIN1; AZI; AZI1; OAZI; AZIA1; OAZIN; ODC1L
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Antizyme Inhibitor 1, Antibody; Antizyme Inhibitor 1 Antibody; Antizyme inhibitor 1; AZI; Azin1; AZIN1_HUMAN; OAZI; OAZIN; ODC1L; Ornithine decarboxylase antizyme inhibitor; anti-AZIN1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Antizyme inhibitor 1 antibody detects endogenous levels of Antizyme inhibitor 1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
448
Applicable Applications for anti-AZIN1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Antizyme inhibitor 1.
Subcellular Location
Nucleus
Tissue Specificity
Expressed in liver.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (91%), Xenopus (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of HEK-293 cells, using Antizyme inhibitor 1 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of HEK-293 cells, using Antizyme inhibitor 1 antibody.)
IHC (Immunohistochemistry)
(AAA323172 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA323172 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-AZIN1 antibody
Function: Antizyme inhibitor (AZI) protein that positively regulates ornithine decarboxylase (ODC) activity and polyamine uptake. AZI is an enzymatically inactive ODC homolog that counteracts the negative effect of ODC antizymes (AZs) OAZ1, OAZ2 and OAZ3 on ODC activity by competing with ODC for antizyme-binding (PubMed:17900240, PubMed:26305948). Inhibits antizyme-dependent ODC degradation and releases ODC monomers from their inactive complex with antizymes, leading to formation of the catalytically active ODC homodimer and restoring polyamine production (PubMed:17900240).
Subunit Structure: Monomer (PubMed:26305948). Interacts with OAZ1 and OAZ3; this interaction disrupts the interaction between the antizyme and ODC1 (Probable).
Post-translational Modifications: Ubiquitinated, leading to its proteasomal degradation; a process that is reduced in presence of antizyme OAZ1.
Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-II family. ODC antizyme inhibitor subfamily.
Subunit Structure: Monomer (PubMed:26305948). Interacts with OAZ1 and OAZ3; this interaction disrupts the interaction between the antizyme and ODC1 (Probable).
Post-translational Modifications: Ubiquitinated, leading to its proteasomal degradation; a process that is reduced in presence of antizyme OAZ1.
Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-II family. ODC antizyme inhibitor subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 49.5 kDa
Predicted: 50 kDa
Predicted: 50 kDa
NCBI Official Full Name
antizyme inhibitor 1 isoform 1
NCBI Official Synonym Full Names
antizyme inhibitor 1
NCBI Official Symbol
AZIN1
NCBI Official Synonym Symbols
AZI; AZI1; OAZI; AZIA1; OAZIN; ODC1L
NCBI Protein Information
antizyme inhibitor 1
UniProt Protein Name
Antizyme inhibitor 1
UniProt Gene Name
AZIN1
UniProt Synonym Gene Names
OAZI; OAZIN; AZI; AZI1
Similar Products
Product Notes
The AZIN1 azin1 (Catalog #AAA323172) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Antizyme Inhibitor 1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Antizyme Inhibitor 1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the AZIN1 azin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Antizyme Inhibitor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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