FCM/FACS (Flow Cytometry)
(ANXA7 Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ANXA7 Polyclonal Antibody | anti-ANXA7 antibody
ANXA7 Antibody (Center)
Gene Names
ANXA7; SNX; ANX7; SYNEXIN
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ANXA7, Antibody; ANXA7 Antibody (Center); Annexin A7; Annexin VII; Annexin-7; Synexin; ANXA7; ANX7; SNX; anti-ANXA7 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ANXA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-356 amino acids from the Central region of human ANXA7.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
329-356
Sequence Length
466
Applicable Applications for anti-ANXA7 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ANXA7 Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ANXA7 Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
((LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with ANXA7 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Immunofluorescence analysis of ANXA7 Antibody (Center) with paraffin-embedded human brain tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI.)
IHC (Immunohiostchemistry)
((LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with ANXA7 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Immunofluorescence analysis of ANXA7 Antibody (Center) with paraffin-embedded human brain tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI.)
WB (Western Blot)
(ANXA7 Antibody (Center) western blot analysis in Jurkat and SH-SY5Y cell lysates (35ug/lane). This demonstrates that the ANXA7 antibody detected ANXA7 protein (arrow).)
WB (Western Blot)
(ANXA7 Antibody (Center) western blot analysis in Jurkat and SH-SY5Y cell lysates (35ug/lane). This demonstrates that the ANXA7 antibody detected ANXA7 protein (arrow).)
Related Product Information for anti-ANXA7 antibody
Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. ANXA7 is a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion.
Product Categories/Family for anti-ANXA7 antibody
References
Shirvan,A., et.al., Biochemistry 33 (22), 6888-6901 (1994)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52739
NCBI Official Full Name
annexin A7 isoform 1
NCBI Official Synonym Full Names
annexin A7
NCBI Official Symbol
ANXA7
NCBI Official Synonym Symbols
SNX; ANX7; SYNEXIN
NCBI Protein Information
annexin A7
UniProt Protein Name
Annexin A7
UniProt Gene Name
ANXA7
UniProt Synonym Gene Names
ANX7; SNX
UniProt Entry Name
ANXA7_HUMAN
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Product Notes
The ANXA7 anxa7 (Catalog #AAA284853) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 329-356. The ANXA7 Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Monkey, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's ANXA7 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ANXA7 anxa7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANXA7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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