IP (Immunoprecipitation)
(Detection of human APC7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-APC7 antibody AAA211920 used for IP at 3 ug/mg lysate. APC7 was also immunoprecipitated by rabbit anti-APC7 antibody which recognizes a downstream epitope. For blotting immunoprecipitated APC7, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human, Mouse APC7 Polyclonal Antibody | anti-APC7 antibody
Rabbit anti-APC7 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
APC7, Antibody; Rabbit anti-APC7 Antibody, Affinity Purified; cyclosome subunit 7; APC7; anaphase-promoting complex subunit 7; Anaphase-promoting complex subunit 7; anaphase promoting complex subunit 7; anti-APC7 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-APC7 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 75 and 125
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human APC7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-APC7 antibody AAA211920 used for IP at 3 ug/mg lysate. APC7 was also immunoprecipitated by rabbit anti-APC7 antibody which recognizes a downstream epitope. For blotting immunoprecipitated APC7, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human APC7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-APC7 antibody AAA211920 used for IP at 3 ug/mg lysate. APC7 was also immunoprecipitated by rabbit anti-APC7 antibody which recognizes a downstream epitope. For blotting immunoprecipitated APC7, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human and mouse APC7 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-APC7 (Cat. No. AAA211920) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human and mouse APC7 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-APC7 (Cat. No. AAA211920) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-APC7 antibody
Anaphase promoting complex subunit 7 (APC7) is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B which prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits.
Product Categories/Family for anti-APC7 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The APC7 anapc7 (Catalog #AAA211920) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-APC7 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's APC7 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the APC7 anapc7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APC7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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