WB (Western Blot)
(Anti-APE1 antibody, AAA45127, Western blottingLane 1: U87 Cell LysateLane 2: A549 Cell LysateLane 3: SMMC Cell LysateLane 4: HELA Cell Lysate)
Rabbit APE1 Polyclonal Antibody | anti-APEX1 antibody
Anti-APE1 antibody
Gene Names
APEX1; APE; APX; APE1; APEN; APEX; HAP1; REF1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
APE1, Antibody; Anti-APE1 antibody; DNA-(apurinic or apyrimidinic site) lyase; APEX nuclease(multifunctional DNA repair enzyme) 1; AP endonuclease 1 antibody; AP endonuclease class I antibody; AP lyase antibody; APE 1 antibody; APE antibody; APE-1 antibody; APEN antibody; APEX 1 antibody; APEX antibody; APEX nuclease(multifunctional DNA repair enzyme) 1 antibody; Apex nuclease 1 antibody; APEX nuclease antibody; APEX1 antibody; APEX1_HUMAN antibody; Apurinic endonuclease antibody; Apurinic-apyrimidinic endonuclease 1 antibody; Apurinic/apyrimidinic(abasic) endonuclease antibody; Apurinic/apyrimidinic endonuclease 1 antibody; Apurinic/apyrimidinic exonuclease antibody; APX antibody; BAP1 antibody; Deoxyribonuclease(apurinic or apyrimidinic) antibody; DNA(apurinic or apyrimidinic site) lyase antibody; DNA-(apurinic or apyrimidinic site) lyase, mitochondrial antibody; EC 4.2.99.18 antibody; HAP 1 antibody; HAP1 antibody; Human Apurinic endonuclease 1 antibody; MGC139790 antibody; Multifunctional DNA repair enzyme antibody; Redox factor 1 antibody; Redox factor-1 antibody; REF 1 antibody; REF 1 protein antibody; REF-1 antibody; REF1 antibody; REF1 protein antibody; anti-APEX1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
318
Applicable Applications for anti-APEX1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human APE1 (177-191aa RGLVRLEYRQRWDEA), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-APE1 antibody, AAA45127, Western blottingLane 1: U87 Cell LysateLane 2: A549 Cell LysateLane 3: SMMC Cell LysateLane 4: HELA Cell Lysate)
WB (Western Blot)
(Anti-APE1 antibody, AAA45127, Western blottingLane 1: U87 Cell LysateLane 2: A549 Cell LysateLane 3: SMMC Cell LysateLane 4: HELA Cell Lysate)
IHC (Immunohiostchemistry)
(Anti-APE1 antibody, AAA45127, IHC(P)IHC(P): Rat Intestine Tissue)
IHC (Immunohiostchemistry)
(Anti-APE1 antibody, AAA45127, IHC(P)IHC(P): Rat Intestine Tissue)
WB (Western Blot)
(Anti-APE1 antibody, AAA45127, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Mouse Brain Tissue Lysate)
WB (Western Blot)
(Anti-APE1 antibody, AAA45127, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Mouse Brain Tissue Lysate)
Related Product Information for anti-APEX1 antibody
Description: Rabbit IgG polyclonal antibody for DNA-(apurinic or apyrimidinic site) lyase(APEX1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: APEX1, also called apurinic endonuclease(APE), is a DNA repair enzyme having apurinic/apyrimidinic(AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.
Background: APEX1, also called apurinic endonuclease(APE), is a DNA repair enzyme having apurinic/apyrimidinic(AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
35,555 Da
NCBI Official Full Name
DNA-(apurinic or apyrimidinic site) lyase
NCBI Official Synonym Full Names
APEX nuclease (multifunctional DNA repair enzyme) 1
NCBI Official Symbol
APEX1
NCBI Official Synonym Symbols
APE; APX; APE1; APEN; APEX; HAP1; REF1
NCBI Protein Information
DNA-(apurinic or apyrimidinic site) lyase; DNA-(apurinic or apyrimidinic site) lyase; AP lyase; protein REF-1; redox factor-1; AP endonuclease class I; apurinic-apyrimidinic endonuclease 1; apurinic/apyrimidinic (abasic) endonuclease; deoxyribonuclease (apurinic or apyrimidinic)
UniProt Protein Name
DNA-(apurinic or apyrimidinic site) lyase
UniProt Gene Name
APEX1
UniProt Synonym Gene Names
APE; APE1; APEX; APX; HAP1; REF1; APEN; AP endonuclease 1; APE-1
UniProt Entry Name
APEX1_HUMAN
Similar Products
Product Notes
The APEX1 apex1 (Catalog #AAA45127) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-APE1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's APE1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the APEX1 apex1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.