FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of A549 cells using anti-APEX2 antibody (AAA125786).Overlay histogram showing A549 cells stained with AAA125786 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APEX2 Antibody (AAA125786,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit APEX2 Polyclonal Antibody | anti-APEX2 antibody
Anti-APEX2 Antibody
Gene Names
APEX2; APE2; XTH2; ZGRF2; APEXL2
Reactivity
Human, Mouse, Rat, Monkey
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
APEX2, Antibody; Anti-APEX2 Antibody; APEX2; APE2; APEXL2; XTH2; DNA-(apurinic or apyrimidinic site lyase 2; EC 3. 1. -. -; EC 4. 2. 99. 18; AP endonuclease XTH2; APEX nuclease 2; APEX nuclease-like 2; Apurinic-apyrimidinic endonuclease 2; AP endonuclease 2; apurinic/apyrimidinic endodeoxyribonuclease 2; anti-APEX2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for APEX2 detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Applicable Applications for anti-APEX2 antibody
ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E Coli-derived human APEX2 recombinant protein (Position: M1-G500).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of A549 cells using anti-APEX2 antibody (AAA125786).Overlay histogram showing A549 cells stained with AAA125786 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APEX2 Antibody (AAA125786,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of A549 cells using anti-APEX2 antibody (AAA125786).Overlay histogram showing A549 cells stained with AAA125786 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APEX2 Antibody (AAA125786,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of APEX2 using anti-APEX2 antibody (AAA125786).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysatesLane 3: human HEK293 whole cell lysatesLane 4: monkey COS-7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-APEX2 antigen affinity purified polyclonal antibody (Catalog # AAA125786) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for APEX2 at approximately 57KD. The expected band size for APEX2 is at 57KD.)
WB (Western Blot)
(Figure 1. Western blot analysis of APEX2 using anti-APEX2 antibody (AAA125786).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysatesLane 3: human HEK293 whole cell lysatesLane 4: monkey COS-7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-APEX2 antigen affinity purified polyclonal antibody (Catalog # AAA125786) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for APEX2 at approximately 57KD. The expected band size for APEX2 is at 57KD.)
Related Product Information for anti-APEX2 antibody
APEX2, also called apurinic/apyrimidinic endonuclease like-2, is a member of the apurinic/apyrimidinic (AP) family of endonucleases that initiate the repair of AP sites formed by spontaneous hydrolysis of the N-glycosylic bond, mutagen-induced base release, or damaged-base excision by a DNA repair glycosylase. RT-PCR detected APEX2 expression in HeLa cells, Jurkat cells, and human kidney, brain and fetal brain tissue. The APEX2 gene is mapped to chromosome Xp11. 21. APEX2 participates in both nuclear and mitochondrial base excision repair (BER) and it can play a role in processing 3-prime-damaged termini or 3-prime-mismatched nucleotides. Additionally, APEX2 displayed weaker AP site-specific and 3-prime nuclease activities compared to APEX1.
References
1. Burkovics, P., Szukacsov, V., Unk, I., Haracska, L. Human Ape2 protein has 3-5-prime exonuclease activity that acts preferentially on mismatched base pairs. Nucleic Acids Res. 34: 2508-2515, 2006.
2. Hadi, M. Z., Ginalski, K., Nguyen, L. H., Wilson, D. M., III. Determinants in nuclease specificity of Ape1 and Ape2, human homologues of Escherichia coli exonuclease III. J. Molec. Biol. 316: 853-866, 2002.
3. Tsuchimoto, D., Sakai, Y., Sakumi, K., Nishioka, K., Sasaki, M., Fujiwara, T., Nakabeppu, Y. Human APE2 protein is mostly localized in the nuclei and to some extent in the mitochondria, while nuclear APE2 is partly associated with proliferating cell nuclear antigen. Nucleic Acids Res. 29: 2349-2360, 2001.
2. Hadi, M. Z., Ginalski, K., Nguyen, L. H., Wilson, D. M., III. Determinants in nuclease specificity of Ape1 and Ape2, human homologues of Escherichia coli exonuclease III. J. Molec. Biol. 316: 853-866, 2002.
3. Tsuchimoto, D., Sakai, Y., Sakumi, K., Nishioka, K., Sasaki, M., Fujiwara, T., Nakabeppu, Y. Human APE2 protein is mostly localized in the nuclei and to some extent in the mitochondria, while nuclear APE2 is partly associated with proliferating cell nuclear antigen. Nucleic Acids Res. 29: 2349-2360, 2001.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57,401 Da
NCBI Official Full Name
DNA-(apurinic or apyrimidinic site) lyase 2
NCBI Official Synonym Full Names
APEX nuclease (apurinic/apyrimidinic endonuclease) 2
NCBI Official Symbol
APEX2
NCBI Official Synonym Symbols
APE2; XTH2; ZGRF2; APEXL2
NCBI Protein Information
DNA-(apurinic or apyrimidinic site) lyase 2; DNA-(apurinic or apyrimidinic site) lyase 2; AP endonuclease 2; AP endonuclease XTH2; zinc finger, GRF-type containing 2; apurinic/apyrimidinic endonuclease-like 2
UniProt Protein Name
DNA-(apurinic or apyrimidinic site) lyase 2
UniProt Gene Name
APEX2
UniProt Synonym Gene Names
APE2; APEXL2; XTH2; AP endonuclease 2
UniProt Entry Name
APEX2_HUMAN
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Product Notes
The APEX2 apex2 (Catalog #AAA125786) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-APEX2 Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's APEX2 can be used in a range of immunoassay formats including, but not limited to, ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the APEX2 apex2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APEX2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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