IHC (Immunohistochemisry)
(APG10L Antibody (C-term S116) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ANKS1B APG10L Antibody (C-term S116) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit APG10L Polyclonal Antibody | anti-ATG10 antibody
APG10L Antibody (C-term S116)
Gene Names
ATG10; APG10; APG10L; pp12616
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
APG10L, Antibody; APG10L Antibody (C-term S116); Ubiquitin-like-conjugating enzyme ATG10; 632-; Autophagy-related protein 10; APG10-like; ATG10; APG10L; anti-ATG10 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This APG10L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-121 amino acids from the C-terminal region of human APG10L.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
96-121
Sequence Length
125
Applicable Applications for anti-ATG10 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(APG10L Antibody (C-term S116) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ANKS1B APG10L Antibody (C-term S116) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(APG10L Antibody (C-term S116) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ANKS1B APG10L Antibody (C-term S116) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of APG10L (arrow) using rabbit polyclonal APG10L Antibody (C-term S116). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG10L gene.)
WB (Western Blot)
(Western blot analysis of APG10L (arrow) using rabbit polyclonal APG10L Antibody (C-term S116). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG10L gene.)
WB (Western Blot)
(APG10L Antibody (C-term S116) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the APG10L antibody detected the APG10L protein (arrow).)
WB (Western Blot)
(APG10L Antibody (C-term S116) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the APG10L antibody detected the APG10L protein (arrow).)
Related Product Information for anti-ATG10 antibody
Autophagy is a process for the bulk degradation of
cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme
involved in 2 ubiquitin-like modifications essential for
autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261)
conjugation and modification of a soluble form of MAP-LC3
(MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a
membrane-bound form (Nemoto et al., 2003 [PubMed
12890687]).
cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme
involved in 2 ubiquitin-like modifications essential for
autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261)
conjugation and modification of a soluble form of MAP-LC3
(MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a
membrane-bound form (Nemoto et al., 2003 [PubMed
12890687]).
Product Categories/Family for anti-ATG10 antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Criollo, A., et al. Cell Death Differ. 14(5):1029-1039(2007)
Shao, Y., et al. Autophagy 3(1):10-16(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Boya, P., et al. Mol. Cell. Biol. 25(3):1025-1040(2005)
Criollo, A., et al. Cell Death Differ. 14(5):1029-1039(2007)
Shao, Y., et al. Autophagy 3(1):10-16(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Boya, P., et al. Mol. Cell. Biol. 25(3):1025-1040(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25279
NCBI Official Full Name
ubiquitin-like-conjugating enzyme ATG10
NCBI Official Synonym Full Names
autophagy related 10
NCBI Official Symbol
ATG10
NCBI Official Synonym Symbols
APG10; APG10L; pp12616
NCBI Protein Information
ubiquitin-like-conjugating enzyme ATG10
UniProt Protein Name
Ubiquitin-like-conjugating enzyme ATG10
UniProt Gene Name
ATG10
UniProt Synonym Gene Names
APG10L; APG10-like
UniProt Entry Name
ATG10_HUMAN
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Product Notes
The ATG10 atg10 (Catalog #AAA287874) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 96-121. The APG10L Antibody (C-term S116) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's APG10L can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ATG10 atg10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APG10L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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