IHC (Immunohistochemisry)
(Cleaved-APG8a/b antibody (MAP1LC3A/B) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Cleaved-APG8a/b antibody (MAP1LC3A/B) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit APG8a/b (MAP1LC3A/B) Polyclonal Antibody | anti-MAP1LC3B antibody
APG8a/b (MAP1LC3A/B) Antibody
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
Immunohistochemistry, Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
APG8a/b (MAP1LC3A/B), Antibody; APG8a/b (MAP1LC3A/B) Antibody; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta; MAP1LC3B; MAP1ALC3; anti-MAP1LC3B antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Cleaved-APG8a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-125 amino acids from human Cleaved-APG8a/b.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
100-125
Sequence Length
125
Applicable Applications for anti-MAP1LC3B antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, IF (Immunofluorescence)
Function
Ubiquitin-like modifier involved in formationof autophagosomal vacuoles(autophagosomes). Plays a role in mitophagywhich contributes to regulate mitochondrialquantity and quality by eliminating themitochondria to a basal level to fulfill cellularenergy requirements and preventing excessROS production (PubMed: ). While LC3sare involved in elongation of the phagophoremembrane, the GABARAP/GATE-16 subfamilyis essential for a later stage inautophagosome maturation. Promotesprimary ciliogenesis by removing OFD1 fromcentriolar satellites via the autophagicpathway. Through its interaction with thereticulophagy receptor TEX264, participatesin the remodeling of subdomains of theendoplasmic reticulum into autophagosomesupon nutrient stress, which then fuse withlysosomes for endoplasmic reticulumturnover (PubMed: , PubMed:31006537).
Cellular Location
Cytoplasm, cytoskeleton. Endomembranesystem; Lipid-anchor. Cytoplasmic vesicle,autophagosome membrane; Lipid- anchor.Note=LC3-II binds to the autophagicmembranes. LC3-II localizes with themitochondrial inner membrane duringParkin-mediated mitophagy(PubMed:28017329). Localizes also todiscrete punctae along the ciliary axoneme.
Tissue Location
Most abundant in heart, brain, skeletalmuscle and testis. Little expression observed in liver
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°Cin small aliquots to prevent freeze-thawcycles.
IHC (Immunohistochemisry)
(Cleaved-APG8a/b antibody (MAP1LC3A/B) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Cleaved-APG8a/b antibody (MAP1LC3A/B) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Cleaved-APG8a/b antibody (MAP1LC3A/B) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Cleaved-APG8a/b antibody (MAP1LC3A/B) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(APG8a/b (MAP1LC3A/B) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the MAP1LC3A antibody detected the MAP1LC3A protein (arrow).)
WB (Western Blot)
(APG8a/b (MAP1LC3A/B) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the MAP1LC3A antibody detected the MAP1LC3A protein (arrow).)
IF (Immunofluorescence)
(Fluorescent image of U251 cells stained with APG8a/b (MAP1LC3A/B) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA290210 APG8a/b (MAP1LC3A/B) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). APG8a/b (MAP1LC3A/B) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
IF (Immunofluorescence)
(Fluorescent image of U251 cells stained with APG8a/b (MAP1LC3A/B) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA290210 APG8a/b (MAP1LC3A/B) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). APG8a/b (MAP1LC3A/B) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
Related Product Information for anti-MAP1LC3B antibody
The product of this gene is a subunit of neuronal
microtubule-associated MAP1A and MAP1B proteins, which are involved
in microtubule assembly and important for neurogenesis. Studies on
the rat homolog implicate a role for this gene in autophagy, a
process that involves the bulk degradation of cytoplasmic
component.
microtubule-associated MAP1A and MAP1B proteins, which are involved
in microtubule assembly and important for neurogenesis. Studies on
the rat homolog implicate a role for this gene in autophagy, a
process that involves the bulk degradation of cytoplasmic
component.
Product Categories/Family for anti-MAP1LC3B antibody
References
References for protein
1.Rouschop, K.M., et al. J. Clin. Invest. 120(1):127-141(2010)
2.Kirkin, V., et al. Mol. Cell 33(4):505-516(2009)
3.Othman, E.Q., et al. J. Clin. Lab. Anal. 23(4):249-258(2009)
4.Liu, Q., et al. Ai Zheng 27(1):25-29(2008)
5.Komatsu, M., et al. Cell 131(6):1149-1163(2007)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].
1.Rouschop, K.M., et al. J. Clin. Invest. 120(1):127-141(2010)
2.Kirkin, V., et al. Mol. Cell 33(4):505-516(2009)
3.Othman, E.Q., et al. J. Clin. Lab. Anal. 23(4):249-258(2009)
4.Liu, Q., et al. Ai Zheng 27(1):25-29(2008)
5.Komatsu, M., et al. Cell 131(6):1149-1163(2007)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
microtubule-associated proteins 1A/1B light chain 3B
NCBI Official Synonym Full Names
microtubule-associated protein 1 light chain 3 beta
NCBI Official Symbol
MAP1LC3B
NCBI Official Synonym Symbols
LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
NCBI Protein Information
microtubule-associated proteins 1A/1B light chain 3B
UniProt Protein Name
Microtubule-associated proteins 1A/1B light chain 3B
UniProt Gene Name
MAP1LC3B
UniProt Synonym Gene Names
MAP1ALC3; MAP1A/MAP1B LC3 B
UniProt Entry Name
MLP3B_HUMAN
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Product Notes
The MAP1LC3B map1lc3b (Catalog #AAA290210) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 100-125. The APG8a/b (MAP1LC3A/B) Antibody reacts with Human, mouse (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's APG8a/b (MAP1LC3A/B) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the MAP1LC3B map1lc3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APG8a/b (MAP1LC3A/B), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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