<
WB (Western Blot)
(Detection of human and mouse API5 by western blot. Samples:Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-API5 antibody AAA212017 (lot AAA212017-2) used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse API5 Polyclonal Antibody | anti-API5 antibody
Rabbit anti-API5 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
API5, Antibody; Rabbit anti-API5 Antibody, Affinity Purified; Fibroblast growth factor 2-interacting factor; Protein XAGL; migration-inducing protein MIG8; AAC11; fibroblast growth factor 2-interacting factor 2; cell migration-inducing gene 8 protein; apoptosis inhibitor 5; API-5; antiapoptosis clone 11 protein; AAC-11; FIF; Apoptosis inhibitor 5; anti-API5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-API5 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
X. laevis, Chicken, Orangutan
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse API5 by western blot. Samples:Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-API5 antibody AAA212017 (lot AAA212017-2) used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse API5 by western blot. Samples:Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-API5 antibody AAA212017 (lot AAA212017-2) used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human API5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-API5 antibody AAA212017 (lot AAA212017-2) used for IP at 3 ug per reaction. API5 was also immunoprecipitated by a previous lot of this antibody (lot AAA212017-1) and rabbit anti-API5 antibody A302-785A. For blotting immunoprecipitated API5, AAA212017 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes)
IP (Immunoprecipitation)
(Detection of human API5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-API5 antibody AAA212017 (lot AAA212017-2) used for IP at 3 ug per reaction. API5 was also immunoprecipitated by a previous lot of this antibody (lot AAA212017-1) and rabbit anti-API5 antibody A302-785A. For blotting immunoprecipitated API5, AAA212017 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes)
IHC (Immunohiostchemistry)
(Detection of mouse API5 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-API5 (Cat. No. AAA212017 Lot2) used at 1:200 (1 ug/ml) Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of mouse API5 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-API5 (Cat. No. AAA212017 Lot2) used at 1:200 (1 ug/ml) Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human API5 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-API5 (Cat. No. AAA212017 Lot2) used at 1:200 (1 ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human API5 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-API5 (Cat. No. AAA212017 Lot2) used at 1:200 (1 ug/ml). Detection: DAB)
Related Product Information for anti-API5 antibody
API5 (apoptosis inhibitor 5) is an antiapoptotic factor that may have a role in protein assembly. API5 negatively regulates ACIN1. By binding to ACIN1, API5 suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. API5 is also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
Product Categories/Family for anti-API5 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The API5 api5 (Catalog #AAA212017) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-API5 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's API5 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the API5 api5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "API5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.