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Application Data
(Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: AIF knockout A549 cell lysate; Predicted MW: 26~35,66kd Observed MW: 66kd Primary Ab: 3ug/ml Rabbit Anti-Human AIF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Rabbit Apoptosis Inducing Factor Polyclonal Antibody | anti-AIF antibody
Polyclonal Antibody to Apoptosis Inducing Factor (AIF)
Gene Names
AIFM1; AIF; CMT2D; CMTX4; COWCK; DFNX5; NADMR; NAMSD; PDCD8; COXPD6
Reactivity
Human, Pig, Bovine
Applications
ELISA, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity Chromatography
Synonyms
Apoptosis Inducing Factor, Antibody; Polyclonal Antibody to Apoptosis Inducing Factor (AIF); anti-AIF antibody
Host
Rabbit
Reactivity
Human, Pig, Bovine
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against AIF. It has beenselected for its ability to recognize AIF in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
0.24mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GLTPEQKQ KKAALSASEG EEVPQDKAPS HVPFLLIGGG TAAFAAARSI RARDPGARVL IVSEDPELPY MRPPLSKELW FSDDPNVTKT LRFKQWNGKE RSIYFQPPSF YVSAQDLPHI ENGGVAVLTG KKVVQLDVRD NMVKLNDGSQ ITYEKCLIAT GGTPRSLSAI DRAGAEVKSR TTLFRKIGDF RSLEKISREV
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GLTPEQKQ KKAALSASEG EEVPQDKAPS HVPFLLIGGG TAAFAAARSI RARDPGARVL IVSEDPELPY MRPPLSKELW FSDDPNVTKT LRFKQWNGKE RSIYFQPPSF YVSAQDLPHI ENGGVAVLTG KKVVQLDVRD NMVKLNDGSQ ITYEKCLIAT GGTPRSLSAI DRAGAEVKSR TTLFRKIGDF RSLEKISREV
Applicable Applications for anti-AIF antibody
ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Fragment
AIF (Gly103~Lys322)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Pig
Conjugate
No Conjugate
Immunogen
Recombinant AIF (Gly103~Lys322) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Application Data
(Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: AIF knockout A549 cell lysate; Predicted MW: 26~35,66kd Observed MW: 66kd Primary Ab: 3ug/ml Rabbit Anti-Human AIF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Application Data
(Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: AIF knockout A549 cell lysate; Predicted MW: 26~35,66kd Observed MW: 66kd Primary Ab: 3ug/ml Rabbit Anti-Human AIF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Stomach Tissue.)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Stomach Tissue.)
IHC (Immunohistochemisry)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
IHC (Immunohistochemisry)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
WB (Western Blot)
(Western Blot: Sample: Recombinant AIF, Human.)
WB (Western Blot)
(Western Blot: Sample: Recombinant AIF, Human.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Porcine Liver Tissue; Lane2: Porcine Heart Tissue; Lane3: Human Hela Cells; Lane4: Human A549 Cells.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Porcine Liver Tissue; Lane2: Porcine Heart Tissue; Lane3: Human Hela Cells; Lane4: Human A549 Cells.)
Product Categories/Family for anti-AIF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,033 Da
NCBI Official Full Name
apoptosis-inducing factor 1, mitochondrial isoform AIFsh
NCBI Official Synonym Full Names
apoptosis inducing factor mitochondria associated 1
NCBI Official Symbol
AIFM1
NCBI Official Synonym Symbols
AIF; CMT2D; CMTX4; COWCK; DFNX5; NADMR; NAMSD; PDCD8; COXPD6
NCBI Protein Information
apoptosis-inducing factor 1, mitochondrial
UniProt Protein Name
Apoptosis-inducing factor 1, mitochondrial
UniProt Gene Name
AIFM1
UniProt Synonym Gene Names
AIF; PDCD8
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Product Notes
The AIF aifm1 (Catalog #AAA142771) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Apoptosis Inducing Factor (AIF) reacts with Human, Pig, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's Apoptosis Inducing Factor can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the AIF aifm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GLTPEQKQ KKAALSASEG EEVPQDKAPS HVPFLLIGGG TAAFAAARSI RARDPGARVL IVSEDPELPY MRPPLSKELW FSDDPNVTKT LRFKQWNGKE RSIYFQPPSF YVSAQDLPHI ENGGVAVLTG KKVVQLDVRD NMVKLNDGSQ ITYEKCLIAT GGTPRSLSAI DRAGAEVKSR TTLFRKIGDF RSLEKISREV. It is sometimes possible for the material contained within the vial of "Apoptosis Inducing Factor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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