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IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Mouse Heart Tissue))
Rabbit anti-Human, Mouse Apoptosis Signal Regulating Kinase 1 (ASK1) Polyclonal Antibody | anti-ASK1 antibody
Polyclonal Antibody to Apoptosis Signal Regulating Kinase 1 (ASK1)
Gene Names
Map3k5; ASK; ASK1; Mekk5; MAPKKK5; 7420452D20Rik
Reactivity
Human, Mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Chromatography
Synonyms
Apoptosis Signal Regulating Kinase 1 (ASK1), Antibody; Polyclonal Antibody to Apoptosis Signal Regulating Kinase 1 (ASK1); anti-ASK1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ASK1. It has been selected for its ability to recognize ASK1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LGKGTYGI VYAGRDLSNQ VRIAIKEIPE RDSRYSQPLH EEIALHKHLK HKNIVQYLGS FSENGFIKIF MEQVPGGSLS ALLRSKWGPL KDNEQTIGFY TKQILEGLKY LHDNQIVHRD IKGDNVLINT YSGVLKISDF GTSKRLAGIN PCTETFTGTL QYMAPEIIDK GPRGYGKAAD IWSLGCTIIE MATGKPPFYE
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LGKGTYGI VYAGRDLSNQ VRIAIKEIPE RDSRYSQPLH EEIALHKHLK HKNIVQYLGS FSENGFIKIF MEQVPGGSLS ALLRSKWGPL KDNEQTIGFY TKQILEGLKY LHDNQIVHRD IKGDNVLINT YSGVLKISDF GTSKRLAGIN PCTETFTGTL QYMAPEIIDK GPRGYGKAAD IWSLGCTIIE MATGKPPFYE
Sequence Length
1374
Applicable Applications for anti-ASK1 antibody
WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry)
Immunogen
Recombinant ASK1 (Leu693~Arg933) expressed in E.coli.
Cross Reactivity
Human, Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Mouse Heart Tissue))
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Mouse Heart Tissue))
IHC (Immunohistochemistry)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
IHC (Immunohistochemistry)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Human 293T cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse ASK1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Human 293T cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse ASK1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Mouse Kidney lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse ASK1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Mouse Kidney lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse ASK1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
154,512 Da
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 5
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 5
NCBI Official Symbol
Map3k5
NCBI Official Synonym Symbols
ASK; ASK1; Mekk5; MAPKKK5; 7420452D20Rik
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 5
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 5
UniProt Gene Name
Map3k5
UniProt Synonym Gene Names
Ask1; Mekk5; ASK-1; MEK kinase 5; MEKK 5
Similar Products
Product Notes
The ASK1 map3k5 (Catalog #AAA135215) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Apoptosis Signal Regulating Kinase 1 (ASK1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Apoptosis Signal Regulating Kinase 1 (ASK1) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the ASK1 map3k5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LGKGTYGI VYAGRDLSNQ VRIAIKEIPE RDSRYSQPLH EEIALHKHLK HKNIVQYLGS FSENGFIKIF MEQVPGGSLS ALLRSKWGPL KDNEQTIGFY TKQILEGLKY LHDNQIVHRD IKGDNVLINT YSGVLKISDF GTSKRLAGIN PCTETFTGTL QYMAPEIIDK GPRGYGKAAD IWSLGCTIIE MATGKPPFYE. It is sometimes possible for the material contained within the vial of "Apoptosis Signal Regulating Kinase 1 (ASK1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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