WB (Western Blot)
(Detection of human ARA70 by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, HeLa, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARA70 antibody (AAA211827 lot 6) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human ARA70 Polyclonal Antibody | anti-ARA70 antibody
Rabbit anti-ARA70 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ARA70, Antibody; Rabbit anti-ARA70 Antibody, Affinity Purified; nuclear receptor coactivator 4; RFG; Ret-activating protein ELE1; RET-activating gene ELE1; ret fused; PTC3; ELE1; ARA70; androgen receptor-associated protein of 70 kDa; Androgen receptor coactivator 70 kDa protein; 70 kDa androgen receptor coactivator; 70 kDa AR-activator; NCoA-4; Nuclear receptor coactivator 4; anti-ARA70 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ARA70 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 564 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ARA70 by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, HeLa, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARA70 antibody (AAA211827 lot 6) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human ARA70 by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, HeLa, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARA70 antibody (AAA211827 lot 6) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ARA70 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARA70 antibody (AAA211827 lot 6) used for IP at 6 ug per reaction. ARA70 was also immunoprecipitated by a previous lot of this antibody (AAA211827 lot 5) and a second antibody against a different epitope of ARA70 . For blotting immunoprecipitated ARA70, AAA211827 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human ARA70 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARA70 antibody (AAA211827 lot 6) used for IP at 6 ug per reaction. ARA70 was also immunoprecipitated by a previous lot of this antibody (AAA211827 lot 5) and a second antibody against a different epitope of ARA70 . For blotting immunoprecipitated ARA70, AAA211827 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-ARA70 antibody
ARA70 (androgen receptor-associated 70) was originally identified as part of chromosomal rearrangement involving the RET proto-oncogene. It was subsequently isolated in a two-hybrid screen as a ligand-dependent associated factor of the androgen receptor (AR). In addition to functioning as a co-activator of the AR, ARA70 has been found to function as a co-activator of the vitamin D receptor, estrogen receptor, PPARalpha, and PPARgamma.
Product Categories/Family for anti-ARA70 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The ARA70 ncoa4 (Catalog #AAA211827) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ARA70 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARA70 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ARA70 ncoa4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARA70, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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