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FCM/FACS (Flow Cytometry)
(ARHGDIA Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ARHGDIA Polyclonal Antibody | anti-ARHGDIA antibody
ARHGDIA Antibody (C-term)
Gene Names
ARHGDIA; GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ARHGDIA, Antibody; ARHGDIA Antibody (C-term); Rho GDP-dissociation inhibitor 1; Rho GDI 1; Rho-GDI alpha; ARHGDIA; GDIA1; anti-ARHGDIA antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ARHGDIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids from the C-terminal region of human ARHGDIA.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
112-140
Sequence Length
204
Applicable Applications for anti-ARHGDIA antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ARHGDIA Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ARHGDIA Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with ARHGDIA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with ARHGDIA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of ARHGDIA (arrow) using rabbit polyclonal ARHGDIA Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARHGDIA gene.)
WB (Western Blot)
(Western blot analysis of ARHGDIA (arrow) using rabbit polyclonal ARHGDIA Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARHGDIA gene.)
WB (Western Blot)
(Western blot analysis of ARHGDIA Antibody (C-term) in A375(lane 1),HL-60(lane 2),Ramos(lane 3) cell line lysates (35ug/lane). ARHGDIA (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of ARHGDIA Antibody (C-term) in A375(lane 1),HL-60(lane 2),Ramos(lane 3) cell line lysates (35ug/lane). ARHGDIA (arrow) was detected using the purified Pab.)
Related Product Information for anti-ARHGDIA antibody
ARHGDIA Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Product Categories/Family for anti-ARHGDIA antibody
References
Qiao,J., etc, Am. J. Physiol., Cell Physiol. 295 (5), C1161-C1168 (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23207
NCBI Official Full Name
rho GDP-dissociation inhibitor 1 isoform a
NCBI Official Synonym Full Names
Rho GDP dissociation inhibitor (GDI) alpha
NCBI Official Symbol
ARHGDIA
NCBI Official Synonym Symbols
GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e
NCBI Protein Information
rho GDP-dissociation inhibitor 1
UniProt Protein Name
Rho GDP-dissociation inhibitor 1
UniProt Gene Name
ARHGDIA
UniProt Synonym Gene Names
GDIA1; Rho GDI 1
UniProt Entry Name
GDIR1_HUMAN
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Product Notes
The ARHGDIA arhgdia (Catalog #AAA288187) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 112-140. The ARHGDIA Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGDIA can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ARHGDIA arhgdia for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGDIA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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