WB (Western Blot)
(Detection of human ARHGDIB/D4-GDI/RhoGDI2 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, HEK293T, CCRF-CEM, HeLa, and HL-60 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARHGDIB/D4-GDI/RhoGDI2 antibody (AAA212955 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human ARHGDIB/D4-GDI/RhoGDI2 Polyclonal Antibody | anti-ARHGDIB/D4-GDI/RhoGDI2 antibody
Rabbit anti-ARHGDIB/D4-GDI/RhoGDI2 Antibody, Affinity Purified
Gene Names
ARHGDIB; D4; GDIA2; GDID4; LYGDI; Ly-GDI; RAP1GN1; RhoGDI2
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ARHGDIB/D4-GDI/RhoGDI2, Antibody; Rabbit anti-ARHGDIB/D4-GDI/RhoGDI2 Antibody, Affinity Purified; LYGDI; RhoGDI2; rho-GDI beta; rho GDP-dissociation inhibitor 2; Rho GDP dissociation inhibitor (GDI) beta; Rho GDI 2; Ly-GDI; GDID4; GDIA2; D4; RAP1GN1; Rho GDP-dissociation inhibitor 2; Rho GDP dissociation inhibitor beta; anti-ARHGDIB/D4-GDI/RhoGDI2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ARHGDIB/D4-GDI/RhoGDI2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 50 and 100
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ARHGDIB/D4-GDI/RhoGDI2 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, HEK293T, CCRF-CEM, HeLa, and HL-60 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARHGDIB/D4-GDI/RhoGDI2 antibody (AAA212955 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human ARHGDIB/D4-GDI/RhoGDI2 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, HEK293T, CCRF-CEM, HeLa, and HL-60 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARHGDIB/D4-GDI/RhoGDI2 antibody (AAA212955 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human ARHGDIB/D4-GDI by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARHGDIB/D4-GDI antibody (AAA212955 lot 2) used for IP at 6 ug per reaction. ARHGDIB/D4-GDI was also immunoprecipitated by a previous lot of this antibody (AAA212955 lot 1) and rabbit anti-ARHGDIB/D4-GDI antibody Brother. For blotting immunoprecipitated ARHGDIB/D4-GDI, AAA212955 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human ARHGDIB/D4-GDI by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARHGDIB/D4-GDI antibody (AAA212955 lot 2) used for IP at 6 ug per reaction. ARHGDIB/D4-GDI was also immunoprecipitated by a previous lot of this antibody (AAA212955 lot 1) and rabbit anti-ARHGDIB/D4-GDI antibody Brother. For blotting immunoprecipitated ARHGDIB/D4-GDI, AAA212955 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human ARHGDIB/D4-GDI by immunohistochemistry. Sample: FFPE section of human breast carcinoma inflammatory cell infiltrate. Antibody: Affinity purified rabbit anti-ARHGDIB/D4-GDI (AAA212955 Lot 2) used at a dilution of 1:5,000 0.2 ug/ml)
IHC (Immunohistochemistry)
(Detection of human ARHGDIB/D4-GDI by immunohistochemistry. Sample: FFPE section of human breast carcinoma inflammatory cell infiltrate. Antibody: Affinity purified rabbit anti-ARHGDIB/D4-GDI (AAA212955 Lot 2) used at a dilution of 1:5,000 0.2 ug/ml)
Related Product Information for anti-ARHGDIB/D4-GDI/RhoGDI2 antibody
ARHGDIB regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to the [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P52566].
Product Categories/Family for anti-ARHGDIB/D4-GDI/RhoGDI2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,988 Da
NCBI Official Full Name
rho GDP-dissociation inhibitor 2
NCBI Official Synonym Full Names
Rho GDP dissociation inhibitor (GDI) beta
NCBI Official Symbol
ARHGDIB
NCBI Official Synonym Symbols
D4; GDIA2; GDID4; LYGDI; Ly-GDI; RAP1GN1; RhoGDI2
NCBI Protein Information
rho GDP-dissociation inhibitor 2; Rho GDI 2; rho-GDI beta
UniProt Protein Name
Rho GDP-dissociation inhibitor 2
UniProt Gene Name
ARHGDIB
UniProt Synonym Gene Names
GDIA2; GDID4; RAP1GN1; Rho GDI 2
UniProt Entry Name
GDIR2_HUMAN
Similar Products
Product Notes
The ARHGDIB/D4-GDI/RhoGDI2 arhgdib (Catalog #AAA212955) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ARHGDIB/D4-GDI/RhoGDI2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGDIB/D4-GDI/RhoGDI2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ARHGDIB/D4-GDI/RhoGDI2 arhgdib for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGDIB/D4-GDI/RhoGDI2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.