FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of PC-3 cells using anti-ARHGEF6 antibody (AAA127289).Overlay histogram showing PC-3 cells stained with AAA127289 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ARHGEF6 Antibody (AAA127289, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Rabbit anti-Human, Mouse ARHGEF6 Polyclonal Antibody | anti-ARHGEF6 antibody
Anti-ARHGEF6 Antibody Picoband
Gene Names
ARHGEF6; PIXA; COOL2; MRX46; Cool-2; alphaPIX; alpha-PIX
Reactivity
Human, Mouse
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
ARHGEF6, Antibody; Anti-ARHGEF6 Antibody Picoband; Rho guanine nucleotide exchange factor 6; Rac/Cdc42 guanine nucleotide exchange factor 6; ARHGEF6; COOL2; KIAA0006; PIXA; Alpha-Pix; COOL-2; PAK-interacting exchange factor alpha; anti-ARHGEF6 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-ARHGEF6 antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E coli-derived human ARHGEF6 recombinant protein (Position: E54-R765). Human ARHGEF6 shares 91.2% and 91.3% amino acid (aa) sequence identity with mouse and rat ARHGEF6, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of PC-3 cells using anti-ARHGEF6 antibody (AAA127289).Overlay histogram showing PC-3 cells stained with AAA127289 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ARHGEF6 Antibody (AAA127289, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of PC-3 cells using anti-ARHGEF6 antibody (AAA127289).Overlay histogram showing PC-3 cells stained with AAA127289 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ARHGEF6 Antibody (AAA127289, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
WB (Western Blot)
(Figure 1. Western blot analysis of ARHGEF6 using anti-ARHGEF6 antibody (AAA127289).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates,Lane 2: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGEF6 antigen affinity purified polyclonal antibody (#AAA127289) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ARHGEF6 at approximately 87 kDa. The expected band size for ARHGEF6 is at 87 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of ARHGEF6 using anti-ARHGEF6 antibody (AAA127289).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates,Lane 2: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGEF6 antigen affinity purified polyclonal antibody (#AAA127289) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ARHGEF6 at approximately 87 kDa. The expected band size for ARHGEF6 is at 87 kDa.)
Related Product Information for anti-ARHGEF6 antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-ARHGEF6 antibody
References
1. Gallo, G., Letourneau, P. C. Axon guidance: GTPases help axons reach their targets. Curr. Biol. 8: R80-R82, 1998.2. Gedeon, A. K., Glass, I. A., Connor, J. M., Mulley, J. C. Genetic localisation of MRX27 to Xq24-26 defines another discrete gene for non-specific X-linked mental retardation. Am. J. Med. Genet. 64: 121-124, 1996.3. Hamosh, A. Personal Communication. Baltimore, Md. 11/21/2018.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
87,499 Da
NCBI Official Full Name
rho guanine nucleotide exchange factor 6
NCBI Official Synonym Full Names
Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6
NCBI Official Symbol
ARHGEF6
NCBI Official Synonym Symbols
PIXA; COOL2; MRX46; Cool-2; alphaPIX; alpha-PIX
NCBI Protein Information
rho guanine nucleotide exchange factor 6; PAK-interacting exchange factor alpha; PAK-interacting exchange factor, alpha; Rac/Cdc42 guanine exchange factor (GEF) 6; rac/Cdc42 guanine nucleotide exchange factor 6
UniProt Protein Name
Rho guanine nucleotide exchange factor 6
UniProt Gene Name
ARHGEF6
UniProt Synonym Gene Names
COOL2; KIAA0006; PIXA
UniProt Entry Name
ARHG6_HUMAN
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Product Notes
The ARHGEF6 arhgef6 (Catalog #AAA127289) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ARHGEF6 Antibody Picoband reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGEF6 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the ARHGEF6 arhgef6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGEF6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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