WB (Western Blot)
(Detection of human ARID4A by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID4A antibody AAA211810 (lot AAA211810-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human ARID4A Polyclonal Antibody | anti-ARID4A antibody
Rabbit anti-ARID4A Antibody, Affinity Purified
Gene Names
ARID4A; RBP1; RBBP1; RBP-1; RBBP-1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ARID4A, Antibody; Rabbit anti-ARID4A Antibody, Affinity Purified; RBP1; RBBP-1; retinoblastoma-binding protein 1; ARID domain-containing protein 4A; AT-rich interactive domain-containing protein 4A; RBBP1; AT rich interactive domain 4A (RBP1-like); RBP-1; AT-rich interaction domain 4A; anti-ARID4A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ARID4A antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 925 and 975
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ARID4A by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID4A antibody AAA211810 (lot AAA211810-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human ARID4A by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID4A antibody AAA211810 (lot AAA211810-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human ARID4A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID4A antibody AAA211810 (lot AAA211810-2) used for IP at 3 ug per reaction. ARID4A was also immunoprecipitated by rabbit anti-ARID4A antibody BL5288. For blotting immunoprecipitated ARID4A, AAA211810 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ARID4A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID4A antibody AAA211810 (lot AAA211810-2) used for IP at 3 ug per reaction. ARID4A was also immunoprecipitated by rabbit anti-ARID4A antibody BL5288. For blotting immunoprecipitated ARID4A, AAA211810 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-ARID4A antibody
ARID4A (AT-rich interactive domain-containing protein 4A) is a retinoblastoma binding protein and member of the AT-rich interaction domain (ARID) family of proteins. In addition to the ARID DNA binding domain, ARID4A also contains a chromo domain and a TUDOR domain, indicating a function in chromatin organization and protein-protein interactions with methylated protein substrates. ARID4A is implicated to be involved in chromatin remodeling and epigenetic modifications and to play a role in the pathogenesis of cancer.
Product Categories/Family for anti-ARID4A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
134,951 Da
NCBI Official Full Name
Homo sapiens AT rich interactive domain 4A (RBP1-like) (ARID4A), transcript variant 1, mRNA
NCBI Official Synonym Full Names
AT rich interactive domain 4A (RBP1-like)
NCBI Official Symbol
ARID4A
NCBI Official Synonym Symbols
RBP1; RBBP1; RBP-1; RBBP-1
NCBI Protein Information
AT-rich interactive domain-containing protein 4A; ARID domain-containing protein 4A; retinoblastoma binding protein 1; retinoblastoma-binding protein 1
UniProt Protein Name
AT-rich interactive domain-containing protein 4A
UniProt Gene Name
ARID4A
UniProt Synonym Gene Names
RBBP1; RBP1; ARID domain-containing protein 4A; RBBP-1
UniProt Entry Name
ARI4A_HUMAN
Similar Products
Product Notes
The ARID4A arid4a (Catalog #AAA211810) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ARID4A Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARID4A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ARID4A arid4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARID4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.