WB (Western Blot)
(ARNT Antibody (V528) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ARNT antibody detected the ARNT protein (arrow).)
Rabbit ARNT Polyclonal Antibody | anti-ARNT antibody
ARNT Antibody (Center V528)
Gene Names
ARNT; HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
Western Blot, ELISA, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ARNT, Antibody; ARNT Antibody (Center V528); Aryl hydrocarbon receptor nuclear translocator; ARNT protein; Class E basic helix-loop-helix protein 2; bHLHe2; Dioxin receptor; nuclear translocator; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-beta; ARNT; BHLHE2; anti-ARNT antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ARNT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-544 amino acids from the Central region of human ARNT.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
513-544
Sequence Length
787
Applicable Applications for anti-ARNT antibody
WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(ARNT Antibody (V528) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ARNT antibody detected the ARNT protein (arrow).)
WB (Western Blot)
(ARNT Antibody (V528) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ARNT antibody detected the ARNT protein (arrow).)
IF (Immunofluorescence)
(Fluorescent image of A549 cell stained with hARNT-V528. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with hARNT primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).hARNT immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent image of A549 cell stained with hARNT-V528. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with hARNT primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).hARNT immunoreactivity is localized to Nucleus significantly.)
Related Product Information for anti-ARNT antibody
The aryl hydrocarbon (Ah) receptor is involved in the
induction of several enzymes that participate in xenobiotic
metabolism. The ligand-free, cytosolic form of the Ah receptor is
complexed to heat shock protein 90. Binding of ligand, which
includes dioxin and polycyclic aromatic hydrocarbons, results in
translocation of the ligand-binding subunit only to the nucleus.
Induction of enzymes involved in xenobiotic metabolism occurs
through binding of the ligand-bound Ah receptor to xenobiotic
responsive elements in the promoters of genes for these enzymes.
This gene encodes a protein that forms a complex with the
ligand-bound Ah receptor, and is required for receptor function.
The encoded protein has also been identified as the beta subunit of
a heterodimeric transcription factor, hypoxia-inducible factor 1. A
t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion
protein, is associated with acute myeloblastic leukemia.
Alternative splicing results in multiple transcript variants.
induction of several enzymes that participate in xenobiotic
metabolism. The ligand-free, cytosolic form of the Ah receptor is
complexed to heat shock protein 90. Binding of ligand, which
includes dioxin and polycyclic aromatic hydrocarbons, results in
translocation of the ligand-binding subunit only to the nucleus.
Induction of enzymes involved in xenobiotic metabolism occurs
through binding of the ligand-bound Ah receptor to xenobiotic
responsive elements in the promoters of genes for these enzymes.
This gene encodes a protein that forms a complex with the
ligand-bound Ah receptor, and is required for receptor function.
The encoded protein has also been identified as the beta subunit of
a heterodimeric transcription factor, hypoxia-inducible factor 1. A
t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion
protein, is associated with acute myeloblastic leukemia.
Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-ARNT antibody
References
Otsubo, K., et al. Cancer Genet. Cytogenet. 202(1):22-26(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Kewley, R.J., et al. Biochem. Biophys. Res. Commun. 338(1):660-667(2005)
Kewley, R.J., et al. Biochem. Biophys. Res. Commun. 338(1):660-667(2005)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Kewley, R.J., et al. Biochem. Biophys. Res. Commun. 338(1):660-667(2005)
Kewley, R.J., et al. Biochem. Biophys. Res. Commun. 338(1):660-667(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
86636
NCBI Official Full Name
aryl hydrocarbon receptor nuclear translocator isoform 4
NCBI Official Synonym Full Names
aryl hydrocarbon receptor nuclear translocator
NCBI Official Symbol
ARNT
NCBI Official Synonym Symbols
HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
NCBI Protein Information
aryl hydrocarbon receptor nuclear translocator
UniProt Protein Name
Aryl hydrocarbon receptor nuclear translocator
UniProt Gene Name
ARNT
UniProt Synonym Gene Names
BHLHE2; ARNT protein; bHLHe2; HIF-1-beta; HIF1-beta
UniProt Entry Name
ARNT_HUMAN
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Product Notes
The ARNT arnt (Catalog #AAA289426) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 513-544. The ARNT Antibody (Center V528) reacts with Human (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's ARNT can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the ARNT arnt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARNT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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