IF (Immunofluorescence)
(Fluorescent confocal image of SK-BR-3 cell stained with ARNT2 Antibody (Center). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARNT2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ARNT2 immunoreactivity is localized to nucleus significantly.)
Rabbit ARNT2 Polyclonal Antibody | anti-ARNT2 antibody
ARNT2 Antibody (Center)
Gene Names
ARNT2; WEDAS; bHLHe1
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ARNT2, Antibody; ARNT2 Antibody (Center); Aryl hydrocarbon receptor nuclear translocator 2; ARNT protein 2; Class E basic helix-loop-helix protein 1; bHLHe1; ARNT2; BHLHE1; KIAA0307; anti-ARNT2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ARNT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-278 amino acids from the Central region of human ARNT2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
249-278
Sequence Length
717
Applicable Applications for anti-ARNT2 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of SK-BR-3 cell stained with ARNT2 Antibody (Center). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARNT2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ARNT2 immunoreactivity is localized to nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of SK-BR-3 cell stained with ARNT2 Antibody (Center). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARNT2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ARNT2 immunoreactivity is localized to nucleus significantly.)
WB (Western Blot)
(ARNT2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ARNT2 antibody detected the ARNT2 protein (arrow).)
WB (Western Blot)
(ARNT2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ARNT2 antibody detected the ARNT2 protein (arrow).)
Related Product Information for anti-ARNT2 antibody
This gene encodes a member of the
basic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily of
transcription factors. The encoded protein acts as a partner for
several sensor proteins of the bHLH-PAS family, forming
heterodimers with the sensor proteins that bind regulatory DNA
sequences in genes responsive to developmental and environmental
stimuli. Under hypoxic conditions, the encoded protein complexes
with hypoxia-inducible factor 1alpha in the nucleus and this
complex binds to hypoxia-responsive elements in enhancers and
promoters of oxygen-responsive genes. A highly similar protein in
mouse forms functional complexes with both aryl hydrocarbon
receptors and Single-minded proteins, suggesting addition roles for
the encoded protein in the metabolism of xenobiotic compounds and
the regulation of neurogenesis, respectively.
basic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily of
transcription factors. The encoded protein acts as a partner for
several sensor proteins of the bHLH-PAS family, forming
heterodimers with the sensor proteins that bind regulatory DNA
sequences in genes responsive to developmental and environmental
stimuli. Under hypoxic conditions, the encoded protein complexes
with hypoxia-inducible factor 1alpha in the nucleus and this
complex binds to hypoxia-responsive elements in enhancers and
promoters of oxygen-responsive genes. A highly similar protein in
mouse forms functional complexes with both aryl hydrocarbon
receptors and Single-minded proteins, suggesting addition roles for
the encoded protein in the metabolism of xenobiotic compounds and
the regulation of neurogenesis, respectively.
Product Categories/Family for anti-ARNT2 antibody
References
Ramirez, J.M., et al. Eur. J. Immunol. 40(9):2450-2459(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Chakrabarti, B., et al. Autism Res 2(3):157-177(2009)
Ferreira, M.A., et al. Nat. Genet. 40(9):1056-1058(2008)
Martinez, V., et al. Breast Cancer Res. Treat. 110(3):521-530(2008)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Chakrabarti, B., et al. Autism Res 2(3):157-177(2009)
Ferreira, M.A., et al. Nat. Genet. 40(9):1056-1058(2008)
Martinez, V., et al. Breast Cancer Res. Treat. 110(3):521-530(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
78691
NCBI Official Full Name
aryl hydrocarbon receptor nuclear translocator 2
NCBI Official Synonym Full Names
aryl-hydrocarbon receptor nuclear translocator 2
NCBI Official Symbol
ARNT2
NCBI Official Synonym Symbols
WEDAS; bHLHe1
NCBI Protein Information
aryl hydrocarbon receptor nuclear translocator 2
UniProt Protein Name
Aryl hydrocarbon receptor nuclear translocator 2
UniProt Gene Name
ARNT2
UniProt Synonym Gene Names
BHLHE1; KIAA0307; ARNT protein 2; bHLHe1
UniProt Entry Name
ARNT2_HUMAN
Similar Products
Product Notes
The ARNT2 arnt2 (Catalog #AAA284017) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 249-278. The ARNT2 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ARNT2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ARNT2 arnt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARNT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.