ICC (Immunocytochemistry)
(Immunocytochemical labeling of Arp2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with Arp2 (C-terminal region) and Arp2 (Thr-237/Thr-238) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to Cy3.)
Rabbit Arp2 Polyclonal Antibody | anti-Arp2 antibody
Arp2 (C-terminal region) Antibody
Gene Names
ACTR2; ARP2
Reactivity
Human, Rat, Mouse, Chicken, Frog
Applications
Immunoprecipitation, Immunocytochemistry, ELISA, Western Blot
Synonyms
Arp2, Antibody; Arp2 (C-terminal region) Antibody; Arp2 (C-terminal region); anti-Arp2 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Chicken, Frog
Clonality
Polyclonal
Specificity
This antibody was affinity purified using Arp2 (C-terminal region) peptide (without carrier). The antibody detects 44 and 32kDa* proteins corresponding to the molecular mass of Arp2 on SDS-PAGE immunoblots of human A431, HUVEC, HeLa, and Jurkat cells as well as rat PC12, mouse C2C12, and rabbit fibroblasts.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ml phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-Arp2 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Arp2 (C-terminal region) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human Arp2. This peptide sequence is highly conserved in rat, mouse, chicken, and frog Arp2 proteins.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Arp2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with Arp2 (C-terminal region) and Arp2 (Thr-237/Thr-238) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to Cy3.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Arp2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with Arp2 (C-terminal region) and Arp2 (Thr-237/Thr-238) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to Cy3.)
WB (Western Blot)
(Western blot of human A431 cells treated with Calyculin A (100nM) for 30 min. Blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with anti-Arp2 (C-terminal) (lanes 1 & 2) or anti-Arp2 (Thr-237/Thr-238) (lanes 3 & 4).)
WB (Western Blot)
(Western blot of human A431 cells treated with Calyculin A (100nM) for 30 min. Blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with anti-Arp2 (C-terminal) (lanes 1 & 2) or anti-Arp2 (Thr-237/Thr-238) (lanes 3 & 4).)
Related Product Information for anti-Arp2 antibody
Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. Arp2 has two phosphosites, Thr-237 and Thr-238, that are evolutionarily conserved, and are phosphorylated along with Tyr-202 in response to growth factor stimulation. These phosphorylation events may regulate binding to the pointed end of actin filaments, and alanine substitutions of these Arp2 phosphosites inhibit membrane protrusions. Thus, phosphorylation may be another mode of Arp2/3 complex regulation in addition to the activity of nucleation-promoting factors.
References
Soderling, S.H. (2009). Sci Signal. 2(55):pe5 (Review).
LeClaire, L.L. et al (2008). J Cell Biol. 182(4):647.
Kelleher, J.F. et al. (1995). J Cell Biol. 131(2):385.
LeClaire, L.L. et al (2008). J Cell Biol. 182(4):647.
Kelleher, J.F. et al. (1995). J Cell Biol. 131(2):385.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,377 Da
NCBI Official Full Name
actin-related protein 2 isoform b
NCBI Official Synonym Full Names
ARP2 actin-related protein 2 homolog (yeast)
NCBI Official Symbol
ACTR2
NCBI Official Synonym Symbols
ARP2
NCBI Protein Information
actin-related protein 2; actin-like protein 2
UniProt Protein Name
Actin-related protein 2
UniProt Gene Name
ACTR2
UniProt Synonym Gene Names
ARP2
UniProt Entry Name
ARP2_HUMAN
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Product Notes
The Arp2 actr2 (Catalog #AAA71567) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Arp2 (C-terminal region) Antibody reacts with Human, Rat, Mouse, Chicken, Frog and may cross-react with other species as described in the data sheet. AAA Biotech's Arp2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Arp2 actr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Arp2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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