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FCM/FACS (Flow Cytometry)
(ARRB1 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ARRB1 Polyclonal Antibody | anti-ARRB1 antibody
ARRB1 Antibody (C-term)
Gene Names
ARRB1; ARB1; ARR1
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rabbit, Rat)
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ARRB1, Antibody; ARRB1 Antibody (C-term); Beta-arrestin-1; Arrestin beta-1; ARRB1; ARR1; anti-ARRB1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rabbit, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ARRB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-363 amino acids from the C-terminal region of human ARRB1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
336-363
Sequence Length
410
Applicable Applications for anti-ARRB1 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ARRB1 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ARRB1 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ARRB1 Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ARRB1 Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(ARRB1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARRB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(ARRB1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARRB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(ARRB1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ARRB1 antibody detected the ARRB1 protein (arrow).)
WB (Western Blot)
(ARRB1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ARRB1 antibody detected the ARRB1 protein (arrow).)
Related Product Information for anti-ARRB1 antibody
Members of arrestin/beta-arrestin protein family are
thought to participate in agonist-mediated desensitization of
G-protein-coupled receptors and cause specific dampening of
cellular responses to stimuli such as hormones, neurotransmitters,
or sensory signals. Arrestin beta 1 is a cytosolic protein and
acts as a cofactor in the beta-adrenergic receptor kinase (BARK)
mediated desensitization of beta-adrenergic receptors. Besides the
central nervous system, it is expressed at high levels in
peripheral blood leukocytes, and thus the BARK/beta-arrestin system
is believed to play a major role in regulating receptor-mediated
immune functions. Alternatively spliced transcripts encoding
different isoforms of arrestin beta 1 have been described, however,
their exact functions are not known.
thought to participate in agonist-mediated desensitization of
G-protein-coupled receptors and cause specific dampening of
cellular responses to stimuli such as hormones, neurotransmitters,
or sensory signals. Arrestin beta 1 is a cytosolic protein and
acts as a cofactor in the beta-adrenergic receptor kinase (BARK)
mediated desensitization of beta-adrenergic receptors. Besides the
central nervous system, it is expressed at high levels in
peripheral blood leukocytes, and thus the BARK/beta-arrestin system
is believed to play a major role in regulating receptor-mediated
immune functions. Alternatively spliced transcripts encoding
different isoforms of arrestin beta 1 have been described, however,
their exact functions are not known.
Product Categories/Family for anti-ARRB1 antibody
References
Shukla, A.K., et al. J. Biol. Chem. 285(39):30115-30125(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Lee, D.K., et al. Biochem. Biophys. Res. Commun. 395(2):185-189(2010)
Kim, J.I., et al. Mol. Cancer Res. 8(4):569-577(2010)
Bjorgo, E., et al. Mol. Cell. Biol. 30(7):1660-1672(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Lee, D.K., et al. Biochem. Biophys. Res. Commun. 395(2):185-189(2010)
Kim, J.I., et al. Mol. Cancer Res. 8(4):569-577(2010)
Bjorgo, E., et al. Mol. Cell. Biol. 30(7):1660-1672(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47066
NCBI Official Full Name
beta-arrestin-1 isoform A
NCBI Official Synonym Full Names
arrestin, beta 1
NCBI Official Symbol
ARRB1
NCBI Official Synonym Symbols
ARB1; ARR1
NCBI Protein Information
beta-arrestin-1
UniProt Protein Name
Beta-arrestin-1
UniProt Gene Name
ARRB1
UniProt Synonym Gene Names
ARR1
UniProt Entry Name
ARRB1_HUMAN
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Product Notes
The ARRB1 arrb1 (Catalog #AAA285234) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 336-363. The ARRB1 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rabbit, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ARRB1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ARRB1 arrb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARRB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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