WB (Western Blot)
(Western blot analysis of extracts from Jurkat , using ASPP2 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit ASPP2 Polyclonal Antibody | anti-ASPP2 antibody
ASPP2 Antibody
Gene Names
TP53BP2; BBP; 53BP2; ASPP2; P53BP2; PPP1R13A
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
ASPP2, Antibody; ASPP2 Antibody; 53BP2; Apoptosis stimulating of p53 protein 2; Apoptosis stimulating protein of p53 2; Apoptosis-stimulating of p53 protein 2; ASPP2; ASPP2_HUMAN; BBP; Bcl2 binding protein; Bcl2-binding protein; NY REN 51 antigen; p53 binding protein 2; p53-binding protein 2; p53BP2; PPP1R13A; Renal carcinoma antigen NY-REN-51; Tp53bp2; Tumor protein p53 binding protein 2; Tumor suppressor p53 binding protein 2; Tumor suppressor p53-binding protein 2; anti-ASPP2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
ASPP2 Antibody detects endogenous levels of total ASPP2.
Tissue Specificity: Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
Tissue Specificity: Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ASPP2 antibody
ELISA (Peptide ELISA), WB (Western Blot)
Immunogen
A synthesized peptide derived from human ASPP2, corresponding to a region within the internal amino acids.
Conjugation
Unconjugated
Fragment
Fab fragment
Subunit Structure
Interacts with P53/TP53; the interaction promotes pro-apototic activity. Interacts with BCL2. Interacts with protein phosphatase 1. Interacts with RELA NF-kappa-B subunit. This interaction probably prevents the activation of apoptosis, possibly by preventing its interaction with TP53. Interacts with APC2 and APPBP1. Interacts with DDX42 (via the C-terminus); the interaction is not inhibited by TP53BP2 ubiquitination and is independent of p53/TP53.
Similarity
The ankyrin repeats and the SH3 domain are required for a specific interactions with TP53.Belongs to the ASPP family.
Subcellular Location
Cytoplasm>Perinuclear region. Nucleus.
Note: Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.
Note: Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from Jurkat , using ASPP2 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat , using ASPP2 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from NIH-3T3 cells, using ASPP2 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from NIH-3T3 cells, using ASPP2 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-ASPP2 antibody
Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions with proteins such as TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
125616 MW
NCBI Official Full Name
apoptosis-stimulating of p53 protein 2 isoform 1
NCBI Official Synonym Full Names
tumor protein p53 binding protein 2
NCBI Official Symbol
TP53BP2
NCBI Official Synonym Symbols
BBP; 53BP2; ASPP2; P53BP2; PPP1R13A
NCBI Protein Information
apoptosis-stimulating of p53 protein 2
UniProt Protein Name
Apoptosis-stimulating of p53 protein 2
UniProt Gene Name
TP53BP2
UniProt Synonym Gene Names
ASPP2; BBP; Bbp; 53BP2; p53-binding protein 2; p53BP2
Similar Products
Product Notes
The ASPP2 tp53bp2 (Catalog #AAA329505) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ASPP2 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's ASPP2 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), WB (Western Blot). Researchers should empirically determine the suitability of the ASPP2 tp53bp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASPP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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