Rabbit anti-Mouse ASXL1 Polyclonal Antibody | anti-ASXL1 antibody

ASXL1 Antibody (Center)

Cat. Num. AAA288414

• Gene Names: ASXL1; MDS; BOPS
• Reactivity: Mouse
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: ASXL1, Antibody; ASXL1 Antibody (Center); Putative Polycomb group protein ASXL1; Additional sex combs-like protein 1; ASXL1; KIAA0978; anti-ASXL1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This ASXL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-549 amino acids from the Central region of human ASXL1.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 521-549
• Sequence Length: 479

• Applicable Applications for anti-ASXL1 antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Probable Polycomb group (PcG) protein involved in transcriptional regulation mediated by ligand-bound nuclear hormone receptors, such as retinoic acid receptors (RARs) and peroxisome proliferator-activated receptor gamma (PPARG) (PubMed:16606617). Acts as a coactivator of RARA and RXRA through association with NCOA1 (PubMed:16606617). Acts as a corepressor for PPARG and suppresses its adipocyte differentiation-inducing activity (By similarity). Non- catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1) (PubMed:20436459, PubMed:30664650, PubMed:36180891). Acts as a sensor of N(6)-methyladenine methylation on DNA (6mA): recognizes and binds 6mA DNA, leading to its ubiquitination and degradation by TRIP12, thereby inactivating the PR-DUB complex and regulating Polycomb silencing (PubMed:30982744). The PR-DUB complex is an epigenetic regulator of gene expression and acts as a transcriptional coactivator, affecting genes involved in development, cell communication, signaling, cell proliferation and cell viability (PubMed:30664650, PubMed:36180891). ASXL1, ASXL2 and ASXL3 function redundantly in the PR-DUB complex (By similarity) (PubMed:30664650). The ASXL proteins are essential for chromatin recruitment and transcriptional activation of associated genes (By similarity). ASXL1 and ASXL2 are important for BAP1 protein stability (PubMed:30664650). Together with BAP1, negatively regulates epithelial-mesenchymal transition (EMT) of trophoblast stem cells during placental development by regulating genes involved in epithelial cell integrity, cell adhesion and cytoskeletal organization (PubMed:34170818).
• Cellular Location: Nucleus.
• Tissue Location: Widely expressed at low level. Expressed in heart, brain, skeletal muscle, placenta, pancreas, spleen, prostate, small intestine, colon, peripheral blood, leukocytes, bone marrow and fetal liver. Highly expressed in testes.
• Preparation and Storage: For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of ASXL1 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

FCM/FACS (Flow Cytometry)

(ASXL1 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(ASXL1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ASXL1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(ASXL1 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the ASXL1 antibody detected the ASXL1 protein (arrow).)

Related Product Information for anti-ASXL1 antibody

This gene is similar to the Drosophila additional sex combs gene, which encodes a chromatin-binding protein required for normal determination of segment identity in the developing embryo. The protein is a member of the Polycomb group of proteins, which are necessary for the maintenance of stable repression of homeotic and other loci. The protein is thought to disrupt chromatin in localized areas, enhancing transcription of certain genes while repressing the transcription of other genes. The protein encoded by this gene functions as a ligand-dependent co-activator for retinoic acid receptor in cooperation with nuclear receptor coactivator 1. Mutations in this gene are associated with myelodysplastic syndromes and chronic myelomonocytic leukemia. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-ASXL1 antibody

Signal Transduction

References

Abdel-Wahab, O., et al. Leukemia 24(9):1656-1657(2010)
Szpurka, H., et al. Leuk. Res. 34(8):969-973(2010)
Sugimoto, Y., et al. Br. J. Haematol. 150(1):83-87(2010)
Boultwood, J., et al. Leukemia 24(6):1139-1145(2010)
Rocquain, J., et al. BMC Cancer 10, 401 (2010) :

NCBI and Uniprot Product Information

• NCBI GI #: 29570782
• NCBI GeneID: 171023
• NCBI Accession #: NP_056153.2
• NCBI GenBank Nucleotide #: NM_015338.5
• UniProt Accession #: Q8IXJ9; Q5JWS9; Q8IYY7; Q9H466; Q9NQF8; Q9UFJ0; Q9UFP8; Q9Y2I4; B2RP59
• Molecular Weight: 165448
• NCBI Official Full Name: putative Polycomb group protein ASXL1 isoform 1
• NCBI Official Synonym Full Names: additional sex combs like transcriptional regulator 1
• NCBI Official Symbol: ASXL1
• NCBI Official Synonym Symbols: MDS; BOPS
• NCBI Protein Information: putative Polycomb group protein ASXL1
• UniProt Protein Name: Putative Polycomb group protein ASXL1
• UniProt Gene Name: ASXL1
• UniProt Synonym Gene Names: KIAA0978
• UniProt Entry Name: ASXL1_HUMAN