IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using ATF2(Phospho-Thr69 or 51) Antibody.)
Rabbit ATF2 Polyclonal Antibody | anti-ATF2 antibody
ATF2 (Phospho-Thr69 or 51) Antibody
Gene Names
ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Synonyms
ATF2, Antibody; ATF2 (Phospho-Thr69 or 51) Antibody; CREB2; CREBP1; anti-ATF2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of ATF-2 only when phosphorylated at threonine 69 or 51.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
505
Applicable Applications for anti-ATF2 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of threonine 69 or 51 (D-Q-T(p)-P-T) derived from Human ATF2.
Modification
Phospho-Thr69 or 51
Target Name
ATF2
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using ATF2(Phospho-Thr69 or 51) Antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using ATF2(Phospho-Thr69 or 51) Antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Phospho-Thr69 or 51) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Phospho-Thr69 or 51) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
WB (Western Blot)
(Western blot analysis of extracts from 3T3 cells untreated or treated with Anisomycin using ATF2(Phospho-Thr69 or 51) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from 3T3 cells untreated or treated with Anisomycin using ATF2(Phospho-Thr69 or 51) Antibody.)
Related Product Information for anti-ATF2 antibody
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.
Product Categories/Family for anti-ATF2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,409 Da
NCBI Official Full Name
cyclic AMP-dependent transcription factor ATF-2 isoform 1
NCBI Official Synonym Full Names
activating transcription factor 2
NCBI Official Symbol
ATF2
NCBI Official Synonym Symbols
HB16; CREB2; TREB7; CREB-2; CRE-BP1
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-2
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-2
UniProt Gene Name
ATF2
UniProt Synonym Gene Names
CREB2; CREBP1; cAMP-dependent transcription factor ATF-2; CREB-2; cAMP-responsive element-binding protein 2
UniProt Entry Name
ATF2_HUMAN
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Product Notes
The ATF2 atf2 (Catalog #AAA306435) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATF2 (Phospho-Thr69 or 51) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATF2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ATF2 atf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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