WB (Western Blot)
(Detection of human ATF6 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ATF6 antibody AAA212272 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ATF6 was also immunoprecipitated by rabbit anti-ATF6 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
Rabbit anti-Human ATF6 Polyclonal Antibody | anti-ATF6 antibody
Rabbit anti-ATF6 Antibody, Affinity Purified
Gene Names
ATF6; ATF6A
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ATF6, Antibody; Rabbit anti-ATF6 Antibody, Affinity Purified; ATF6-alpha; cAMP-dependent transcription factor ATF-6 alpha; Activating transcription factor 6 alpha; ACHM7; cyclic AMP-dependent transcription factor ATF-6 alpha; ATF6A; Cyclic AMP-dependent transcription factor ATF-6 alpha; activating transcription factor 6; anti-ATF6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ATF6 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 620 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ATF6 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ATF6 antibody AAA212272 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ATF6 was also immunoprecipitated by rabbit anti-ATF6 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
WB (Western Blot)
(Detection of human ATF6 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ATF6 antibody AAA212272 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ATF6 was also immunoprecipitated by rabbit anti-ATF6 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human ATF6 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ATF6 (Cat. No. AAA212272 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human ATF6 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ATF6 (Cat. No. AAA212272 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).)
Related Product Information for anti-ATF6 antibody
Activating transcription factor 6 (ATF6) is a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells [taken from NCBI Entrez Gene (Gene ID: 22926)].
Product Categories/Family for anti-ATF6 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,585 Da
NCBI Official Full Name
Homo sapiens activating transcription factor 6 (ATF6), mRNA
NCBI Official Synonym Full Names
activating transcription factor 6
NCBI Official Symbol
ATF6
NCBI Official Synonym Symbols
ATF6A
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 alpha
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-6 alpha
UniProt Gene Name
ATF6
UniProt Synonym Gene Names
cAMP-dependent transcription factor ATF-6 alpha; ATF6-alpha
UniProt Entry Name
ATF6A_HUMAN
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Product Notes
The ATF6 atf6 (Catalog #AAA212272) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ATF6 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATF6 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ATF6 atf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATF6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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