WB (Western Blot)
(Western blot analysis of extracts from Serum-starved EC304 cells, using Phospho-Atg14 (Ser29) Antibody. The lane on the left was treated with blocking peptide.)
Rabbit anti-Human, Mouse Atg14 Polyclonal Antibody | anti-Atg14 antibody
Phospho-Atg14 (Ser29) Antibody
Gene Names
ATG14; ATG14L; BARKOR; KIAA0831
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Synonyms
Atg14, Antibody; Phospho-Atg14 (Ser29) Antibody; 4832427M01; ATG14; Atg14L; Autophagy-related protein 14-like protein; BAKOR_HUMAN; Barkor; Beclin 1-associated autophagy-related key regulator; D14Ertd114e; D14Ertd436e; KIAA0831; mCG_6911; anti-Atg14 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Phospho-Atg14 (Ser29) Antibody detects endogenous levels of Atg14 only when phosphorylated at Ser29.
Purity/Purification
Peptide affinity purification
Form/Format
IgG in phosphate buffered saline , pH 7.4, 150mMNaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Atg14 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Atg14 aroundthe phosphorylation site of Ser29.
Preparation and Storage
Store at -20°C.Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from Serum-starved EC304 cells, using Phospho-Atg14 (Ser29) Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Serum-starved EC304 cells, using Phospho-Atg14 (Ser29) Antibody. The lane on the left was treated with blocking peptide.)
IHC (Immunohistochemistry)
(AAA321304 at 1/100 staining Mouse skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(AAA321304 at 1/100 staining Mouse skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Related Product Information for anti-Atg14 antibody
Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1. Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65kDa
NCBI Official Full Name
beclin 1-associated autophagy-related key regulator
NCBI Official Synonym Full Names
autophagy related 14
NCBI Official Symbol
ATG14
NCBI Official Synonym Symbols
ATG14L; BARKOR; KIAA0831
NCBI Protein Information
beclin 1-associated autophagy-related key regulator
UniProt Protein Name
Beclin 1-associated autophagy-related key regulator
UniProt Gene Name
ATG14
UniProt Synonym Gene Names
ATG14L1 PublicationManual assertion based on opinion iniRef.10; Barkor1 PublicationManual assertion based on opinion iniRef.8; Atg14L1 PublicationManual assertion based on opinion iniRef.10
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Product Notes
The Atg14 atg14 (Catalog #AAA321304) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Atg14 (Ser29) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Atg14 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Atg14 atg14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Atg14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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