Rabbit ATP1B2 Polyclonal Antibody | anti-ATP1B2 antibody

ATP1B2 Antibody (Center)

Cat. Num. AAA290948

• Gene Names: ATP1B2; AMOG
• Reactivity: Human, Mouse; Predicted: Bovine, Rabbit, Rat
• Applications: Immunohistochemistry, Dot Blot, ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: ATP1B2, Antibody; ATP1B2 Antibody (Center); ATP1B2; Sodium/potassium-transporting ATPase subunit beta-2; Sodium/potassium-dependent ATPase subunit beta-2; anti-ATP1B2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Bovine, Rabbit, Rat
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This ATP1B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-141 amino acids from the Central region of human ATP1B2.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 290

• Applicable Applications for anti-ATP1B2 antibody: IHC (Immunohistochemistry), DB (Dot Blot), ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
• Antigen Source: Human.
• Cellular Location: Cell membrane; Single-pass type II membrane protein
• Function: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known.
• Antigen Region: 115-141.
• Function: This is the non-catalytic component of the active-enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na (+) and K (+)ions across the plasma membrane. The exact function of the beta-2 subunit is not known.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(ATP1B2 Antibody (Center) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ATP1B2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of lysates from human brain,mouse brain and human cerebellum tissue lysate (from left to right), using ATP1B2 Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

Related Product Information for anti-ATP1B2 antibody

Background: The protein belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.

References

Guey,L.T., et.al., Eur. Urol. 57 (2), 283-292 (2010). Tokhtaeva,E., et.al., Biochemistry 48 (48), 11421-11431 (2009). Hosgood,H.D. et.al., Respir Med 103 (12), 1866-1870 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 49574491
• NCBI GeneID: 482
• NCBI Accession #: NP_001669.3
• NCBI GenBank Nucleotide #: NP_001669.3
• UniProt Accession #: P14415; P13638; Q8WMG3; P14231; Q28030
• Molecular Weight: Human=33; Mouse=33; Rat=33 KDa
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit beta-2 isoform 1
• NCBI Official Synonym Full Names: ATPase Na+/K+ transporting subunit beta 2
• NCBI Official Symbol: ATP1B2
• NCBI Official Synonym Symbols: AMOG
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit beta-2
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit beta-2
• UniProt Gene Name: ATP1B2
• UniProt Synonym Gene Names: AMOG
• UniProt Entry Name: AT1B2_HUMAN