Rabbit ATP5B Polyclonal Antibody | anti-ATP5B antibody

ATP5B Antibody (Center)

Cat. Num. AAA28724

• Gene Names: ATP5B; ATPMB; ATPSB; HEL-S-271
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Rat)
• Applications: ELISA, Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ATP5B, Antibody; ATP5B Antibody (Center); ATP synthase subunit beta; mitochondrial; ATP5B; ATPMB; ATPSB; anti-ATP5B antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ATP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-163 amino acids from the Central region of human ATP5B.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.398 (varies by lot)
• Sequence Positions: 135-163
• Sequence Length: 529

• Applicable Applications for anti-ATP5B antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: IHC~~1:50~100; IF~~1:25; WB~~1:1000
• Antigen Type: Synthetic Peptide
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry)

(ATP5B Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistchemistry)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with ATP5B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of ATP5B (arrow) using rabbit polyclonal ATP5B Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATP5B gene (Lane 2).)

WB (Western Blot)

(Western blot analysis of ATP5B Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate. ATP5B (arrow) was detected using the purified Pab.)

IF (Immunofluorescence)

(Fluorescent image of SK-BR-3 cells stained with ATP5B Antibody (Center). AAA28724 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded H. small intestine section using ATP5B Antibody (Center). AAA28724 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded H. liver section using ATP5B Antibody (Center). AAA28724 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

Related Product Information for anti-ATP5B antibody

ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). It is the beta subunit of the catalytic core.

Product Categories/Family for anti-ATP5B antibody

Crown Antibodies; Metabolism; Signal Transduction

References

Neckelmann,N., et.al., Genomics 5 (4), 829-843 (1989) Ohta,S., et.al., J. Biol. Chem. 263 (23), 11257-11262 (1988) Wallace,D.C., et.al., Curr. Genet. 12 (2), 81-90 (1987)

NCBI and Uniprot Product Information

• NCBI GI #: 32189394
• NCBI GeneID: 506
• NCBI Accession #: NP_001677.2
• NCBI GenBank Nucleotide #: NM_001686.3
• UniProt Accession #: P06576; Q14283; A8K4X0
• Molecular Weight: 56560
• NCBI Official Full Name: ATP synthase subunit beta, mitochondrial
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
• NCBI Official Symbol: ATP5B
• NCBI Official Synonym Symbols: ATPMB; ATPSB; HEL-S-271
• NCBI Protein Information: ATP synthase subunit beta, mitochondrial
• UniProt Protein Name: ATP synthase subunit beta, mitochondrial
• UniProt Gene Name: ATP5B
• UniProt Synonym Gene Names: ATPMB; ATPSB
• UniProt Entry Name: ATPB_HUMAN