WB (Western Blot)
(Detection of human and mouse ATP5O by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATP5O antibody AAA213258 (lot AAA213258-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human, Mouse ATP5O Polyclonal Antibody | anti-ATP5O antibody
Rabbit anti-ATP5O Antibody, Affinity Purified
Gene Names
ATP5O; ATPO; OSCP
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ATP5O, Antibody; Rabbit anti-ATP5O Antibody, Affinity Purified; ATPO; OSCP; oligomycin sensitivity conferring protein; oligomycin sensitivity conferral protein oscp-like protein; Oligomycin sensitivity conferral protein; HMC08D05; ATP5O; ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit; ATP synthase subunit O, mitochondrial; ATP synthase peripheral stalk subunit OSCP; human ATP synthase OSCP subunit; anti-ATP5O antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ATP5O antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 163 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse ATP5O by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATP5O antibody AAA213258 (lot AAA213258-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human and mouse ATP5O by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATP5O antibody AAA213258 (lot AAA213258-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human ATP5O by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATP5O antibody AAA213258 (lot AAA213258-1) used for IP at 6 ug per reaction. ATP5O was also immunoprecipitated by rabbit anti-ATP5O antibody For blotting immunoprecipitated ATP5O, AAA213258 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human ATP5O by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATP5O antibody AAA213258 (lot AAA213258-1) used for IP at 6 ug per reaction. ATP5O was also immunoprecipitated by rabbit anti-ATP5O antibody For blotting immunoprecipitated ATP5O, AAA213258 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-ATP5O antibody
ATP5O is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. ATP5O appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance [taken from NCBI Entrez Gene (Gene ID: 539)].
Product Categories/Family for anti-ATP5O antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,277 Da
NCBI Official Full Name
ATP synthase subunit O, mitochondrial
NCBI Official Synonym Full Names
ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
NCBI Official Symbol
ATP5O
NCBI Official Synonym Symbols
ATPO; OSCP
NCBI Protein Information
ATP synthase subunit O, mitochondrial; human ATP synthase OSCP subunit; oligomycin sensitivity conferral protein; oligomycin sensitivity conferring protein
UniProt Protein Name
ATP synthase subunit O, mitochondrial
UniProt Gene Name
ATP5O
UniProt Synonym Gene Names
ATPO; OSCP
UniProt Entry Name
ATPO_HUMAN
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Product Notes
The ATP5O atp5o (Catalog #AAA213258) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ATP5O Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATP5O can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ATP5O atp5o for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP5O, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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