FCM/FACS (Flow Cytometry)
(ATP6V0A4 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human ATP6V0A4 Polyclonal Antibody | anti-ATP6V0A4 antibody
ATP6V0A4 Antibody (Center)
Gene Names
ATP6V0A4; A4; STV1; VPH1; VPP2; RTA1C; RTADR; ATP6N2; RDRTA2; ATP6N1B
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ATP6V0A4, Antibody; ATP6V0A4 Antibody (Center); V-type proton ATPase 116 kDa subunit a isoform 4; V-ATPase 116 kDa isoform a4; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4; Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform; ATP6V0A4; ATP6N1B; ATP6N2; anti-ATP6V0A4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ATP6V0A4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-261 amino acids from the Central region of human ATP6V0A4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
235-261
Sequence Length
840
Applicable Applications for anti-ATP6V0A4 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ATP6V0A4 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ATP6V0A4 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(ATP6V0A4 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ATP6V0A4 antibody detected the ATP6V0A4 protein (arrow).)
WB (Western Blot)
(ATP6V0A4 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ATP6V0A4 antibody detected the ATP6V0A4 protein (arrow).)
Related Product Information for anti-ATP6V0A4 antibody
This gene encodes a component of vacuolar ATPase
(V-ATPase), a multisubunit enzyme that mediates acidification of
intracellular compartments of eukaryotic cells. V-ATPase dependent
acidification is necessary for such intracellular processes as
protein sorting, zymogen activation, receptor-mediated endocytosis,
and synaptic vesicle proton gradient generation. V-ATPase is
composed of a cytosolic V1 domain and a transmembrane V0 domain.
The V1 domain consists of three A and three B subunits, two G
subunits plus the C, D, E, F, and H subunits. The V1 domain
contains the ATP catalytic site. The V0 domain consists of five
different subunits: a, c, c', c'', and d. This gene is one of four
genes in man and mouse that encode different isoforms of the a
subunit. Alternatively spliced transcript variants encoding the
same protein have been described. Mutations in this gene are
associated with renal tubular acidosis associated with preserved
hearing.
(V-ATPase), a multisubunit enzyme that mediates acidification of
intracellular compartments of eukaryotic cells. V-ATPase dependent
acidification is necessary for such intracellular processes as
protein sorting, zymogen activation, receptor-mediated endocytosis,
and synaptic vesicle proton gradient generation. V-ATPase is
composed of a cytosolic V1 domain and a transmembrane V0 domain.
The V1 domain consists of three A and three B subunits, two G
subunits plus the C, D, E, F, and H subunits. The V1 domain
contains the ATP catalytic site. The V0 domain consists of five
different subunits: a, c, c', c'', and d. This gene is one of four
genes in man and mouse that encode different isoforms of the a
subunit. Alternatively spliced transcript variants encoding the
same protein have been described. Mutations in this gene are
associated with renal tubular acidosis associated with preserved
hearing.
Product Categories/Family for anti-ATP6V0A4 antibody
References
Andreucci, E., et al. Pediatr. Nephrol. 24(11):2147-2153(2009)
Hinton, A., et al. J. Biol. Chem. 284(24):16400-16408(2009)
Su, Y., et al. Am. J. Physiol. Renal Physiol. 295 (4), F950-F958 (2008) :
Norgett, E.E., et al. J. Biol. Chem. 282(19):14421-14427(2007)
Smith, A.N., et al. J. Am. Soc. Nephrol. 16(5):1245-1256(2005)
Forgac, M. J. Biol. Chem. 274(19):12951-12954(1999)
Nelson, N., et al. Physiol. Rev. 79(2):361-385(1999)
Kane, P.M. J. Bioenerg. Biomembr. 31(1):3-5(1999)
Finbow, M.E., et al. Biochem. J. 324 (PT 3), 697-712 (1997) :
Stevens, T.H., et al. Annu. Rev. Cell Dev. Biol. 13, 779-808 (1997) :
Hinton, A., et al. J. Biol. Chem. 284(24):16400-16408(2009)
Su, Y., et al. Am. J. Physiol. Renal Physiol. 295 (4), F950-F958 (2008) :
Norgett, E.E., et al. J. Biol. Chem. 282(19):14421-14427(2007)
Smith, A.N., et al. J. Am. Soc. Nephrol. 16(5):1245-1256(2005)
Forgac, M. J. Biol. Chem. 274(19):12951-12954(1999)
Nelson, N., et al. Physiol. Rev. 79(2):361-385(1999)
Kane, P.M. J. Bioenerg. Biomembr. 31(1):3-5(1999)
Finbow, M.E., et al. Biochem. J. 324 (PT 3), 697-712 (1997) :
Stevens, T.H., et al. Annu. Rev. Cell Dev. Biol. 13, 779-808 (1997) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
96386
NCBI Official Full Name
V-type proton ATPase 116 kDa subunit a isoform 4
NCBI Official Synonym Full Names
ATPase, H+ transporting, lysosomal V0 subunit a4
NCBI Official Symbol
ATP6V0A4
NCBI Official Synonym Symbols
A4; STV1; VPH1; VPP2; RTA1C; RTADR; ATP6N2; RDRTA2; ATP6N1B
NCBI Protein Information
V-type proton ATPase 116 kDa subunit a isoform 4
UniProt Protein Name
V-type proton ATPase 116 kDa subunit a isoform 4
UniProt Gene Name
ATP6V0A4
UniProt Synonym Gene Names
ATP6N1B; ATP6N2; V-ATPase 116 kDa isoform a4
UniProt Entry Name
VPP4_HUMAN
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Product Notes
The ATP6V0A4 atp6v0a4 (Catalog #AAA290181) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 235-261. The ATP6V0A4 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATP6V0A4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ATP6V0A4 atp6v0a4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP6V0A4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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