Rabbit ATP6V0C / VATL Polyclonal Antibody | anti-ATP6V0C antibody

Anti-ATP6V0C / VATL Antibody (aa100-126) IHC-plus

Cat. Num. AAA162137

• Gene Names: ATP6V0C; ATPL; VATL; VPPC; Vma3; ATP6C; ATP6L
• Reactivity: Mouse, Human; Predicted Reactivity: Rat, Bovine, Drosophila, C. elegans
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A Purified
• Synonyms: ATP6V0C / VATL, Antibody; Anti-ATP6V0C / VATL Antibody (aa100-126) IHC-plus; Rabbit Polyclonal to Human ATP6V0C / VATL; Human ATP6V0C / VATL; ATPL; VATL; Vma3; VPPC; ATP6C; ATP6L; anti-ATP6V0C antibody

• Host: Rabbit
• Reactivity: Mouse, Human; Predicted Reactivity: Rat, Bovine, Drosophila, C. elegans
• Clonality: Polyclonal
• Specificity: This ATP6V0C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-126 amino acids from the C-terminal region of human ATP6V0C.
• Purity/Purification: Protein A Purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 155

• Applicable Applications for anti-ATP6V0C antibody: WB (Western Blot), IHC (Immunohistochemistry)
• Immunogen Species: ATP6V0C / VATL antibody was raised against Human
• Epitope: aa100-126
• Family/Subfamily: Transporter / ATPase - V type
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months.; For long term storage store at -20 degree C.

WB (Western Blot)

(ATP6V0C Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The ATP6V0C antibody detected the ATP6V0C protein (arrow).)

IHC (Immunohistochemistry)

(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-ATP6V0C antibody

ATP6V0C Antibody, ATPL Antibody, VATL Antibody, Vma3 Antibody, VPPC Antibody, ATP6C Antibody, ATP6L Antibody Description: ATP6V0C / VATL is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain.

NCBI and Uniprot Product Information

• NCBI GI #: 4502313
• NCBI GeneID: 527
• NCBI Accession #: NP_001685.1
• NCBI GenBank Nucleotide #: NM_001694.3
• UniProt Accession #: P27449; Q6FH26
• NCBI Official Full Name: V-type proton ATPase 16 kDa proteolipid subunit
• NCBI Official Synonym Full Names: ATPase H+ transporting V0 subunit c
• NCBI Official Symbol: ATP6V0C
• NCBI Official Synonym Symbols: ATPL; VATL; VPPC; Vma3; ATP6C; ATP6L
• NCBI Protein Information: V-type proton ATPase 16 kDa proteolipid subunit
• UniProt Protein Name: V-type proton ATPase 16 kDa proteolipid subunit
• UniProt Gene Name: ATP6V0C
• UniProt Synonym Gene Names: ATP6C; ATP6L; ATPL; V-ATPase 16 kDa proteolipid subunit
• UniProt Entry Name: VATL_HUMAN