WB (Western Blot)
(Detection of human and mouse ATP6V1A by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATP6V1A antibody AAA213143 (lot AAA213143-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse ATP6V1A Polyclonal Antibody | anti-ATP6V1A antibody
Rabbit anti-ATP6V1A Antibody, Affinity Purified
Gene Names
ATP6V1A; HO68; VA68; VPP2; Vma1; ATP6A1; ATP6V1A1
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ATP6V1A, Antibody; Rabbit anti-ATP6V1A Antibody, Affinity Purified; vacuolar proton pump alpha subunit 1; vacuolar proton pump subunit alpha; V-ATPase 69 kDa subunit; V-ATPase 69 kDa subunit 1; V-ATPase A subunit 1; V-ATPase subunit A; Vma1; V-type proton ATPase catalytic subunit A; H(+)-transporting two-sector ATPase, subunit A; vacuolar ATPase isoform VA68; VPP2; ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A; HO68; vacuolar ATP synthase catalytic subunit A, ubiquitous isoform; ATP6V1A1; ATPase, H+ transporting, lysosomal, subunit A1; H+-transporting ATPase chain A, vacuolar (VA68 type); ARCL2D; IECEE3; VA68; ATP6A1; ATPase H+ transporting V1 subunit A; anti-ATP6V1A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ATP6V1A antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 567 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse ATP6V1A by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATP6V1A antibody AAA213143 (lot AAA213143-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse ATP6V1A by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATP6V1A antibody AAA213143 (lot AAA213143-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ATP6V1A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATP6V1A antibody AAA213143 (lot AAA213143-1) used for IP at 6 ug per reaction. ATP6V1A was also immunoprecipitated by rabbit anti-ATP6V1A antibody For blotting immunoprecipitated ATP6V1A, AAA213143 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ATP6V1A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATP6V1A antibody AAA213143 (lot AAA213143-1) used for IP at 6 ug per reaction. ATP6V1A was also immunoprecipitated by rabbit anti-ATP6V1A antibody For blotting immunoprecipitated ATP6V1A, AAA213143 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-ATP6V1A antibody
The gene ATP6V1A encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The component, V-type proton ATPase catalytic subunit A, is one of two V1 domain A subunit isoforms and is found in all tissues [taken from NCBI Entrez Gene (Gene ID: 523)].
Product Categories/Family for anti-ATP6V1A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,736 Da
NCBI Official Full Name
V-type proton ATPase catalytic subunit A
NCBI Official Synonym Full Names
ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
NCBI Official Symbol
ATP6V1A
NCBI Official Synonym Symbols
HO68; VA68; VPP2; Vma1; ATP6A1; ATP6V1A1
NCBI Protein Information
V-type proton ATPase catalytic subunit A; V-ATPase subunit A; V-ATPase A subunit 1; V-ATPase 69 kDa subunit 1; vacuolar ATPase isoform VA68; vacuolar proton pump subunit alpha; vacuolar proton pump alpha subunit 1; ATPase, H+ transporting, lysosomal, subu
UniProt Protein Name
V-type proton ATPase catalytic subunit A
UniProt Gene Name
ATP6V1A
UniProt Synonym Gene Names
ATP6A1; ATP6V1A1; VPP2; V-ATPase subunit A
UniProt Entry Name
VATA_HUMAN
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Product Notes
The ATP6V1A atp6v1a (Catalog #AAA213143) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ATP6V1A Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATP6V1A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ATP6V1A atp6v1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP6V1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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