Rabbit anti-Human ATP7A Polyclonal Antibody | anti-ATP7A antibody

Anti-ATP7A Antibody Picoband

Cat. Num. AAA127033

• Gene Names: ATP7A; MK; MNK; DSMAX; SMAX3
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: ATP7A, Antibody; Anti-ATP7A Antibody Picoband; Copper-transporting ATPase 1; ATPase copper transporting alpha; ATP7A; MC1; MNK; EC 7.2.2.8; Copper pump 1; Menkes disease-associated protein; anti-ATP7A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Applicable Applications for anti-ATP7A antibody: FCM/FACS (Flow Cytometry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ATP7A, which shares 79.2% amino acid (aa) sequence identity with both mouse and rat ATP7A.
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
• Cross Reactivity: No cross-reactivity with other proteins
• Preparation and Storage: At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

FCM/FACS (Flow Cytometry)

(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-ATP7A antibody (AAA127033).Overlay histogram showing HepG2 cells stained with AAA127033 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP7A Antibody (AAA127033, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

WB (Western Blot)

(Figure 1. Western blot analysis of ATP7A using anti-ATP7A antibody (AAA127033).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human SH-SY5Y whole cell lysates,Lane 3: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP7A antigen affinity purified polyclonal antibody (#AAA127033) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATP7A at approximately 163 kDa. The expected band size for ATP7A is at 163 kDa.)

Related Product Information for anti-ATP7A antibody

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Categories/Family for anti-ATP7A antibody

Primary Antibodies

References

1. Cecchi, C., Biasotto, M., Tosi, M., Avner, P. The mottled mouse as a model for human Menkes disease: identification of mutations in the Atp7a gene. Hum. Molec. Genet. 6: 425-433, 1997. Note: Erratum: Hum. Molec. Genet. 6: 829 only, 1997.2. Chelly, J., Tumer, Z., Tonnesen, T., Petterson, A., Ishikawa-Brush, Y., Tommerup, N., Horn, N., Monaco, A. P. Isolation of a candidate gene for Menkes disease that encodes a potential heavy metal binding protein. Nature Genet. 3: 14-19, 1993.3. Christodoulou, J., Danks, D. M., Sarkar, B., Baerlocher, K. E., Casey, R., Horn, N., Tumer, Z., Clarke, J. T. R. Early treatment of Menkes disease with parenteral cooper (sic)-histidine: long-term follow-up of four treated patients. Am. J. Med. Genet. 76: 154-164, 1998.

NCBI and Uniprot Product Information

• NCBI GI #: 115529486
• NCBI GeneID: 538
• NCBI Accession #: NP_000043.3
• NCBI GenBank Nucleotide #: NM_000052.5
• UniProt Accession #: Q04656; O00227; O00745; Q9BYY8; B1AT72
• Molecular Weight: 11,522 Da
• NCBI Official Full Name: copper-transporting ATPase 1
• NCBI Official Synonym Full Names: ATPase, Cu++ transporting, alpha polypeptide
• NCBI Official Symbol: ATP7A
• NCBI Official Synonym Symbols: MK; MNK; DSMAX; SMAX3
• NCBI Protein Information: copper-transporting ATPase 1; copper pump 1; Cu++-transporting P-type ATPase; Menkes disease-associated protein
• UniProt Protein Name: Copper-transporting ATPase 1
• UniProt Gene Name: ATP7A
• UniProt Synonym Gene Names: MC1; MNK
• UniProt Entry Name: ATP7A_HUMAN